RP-HPLC法测定小建中片中桂皮醛的含量  

Content Determination of Cinnamaldehyde in Xiaojianzhong Tablet by RP-HPLC

作　　者：许亚玲[1] 罗曼[1] 周兰[1] 申璀[1] 

机构地区：[1]贵州省食品药品检验所,贵阳550004

出　　处：《中国药房》2012年第32期3044-3046,共3页China Pharmacy

基　　金：2010-2011年度国家药品标准提高研究课题(261)

摘　　要：目的:建立测定小建中片中桂皮醛含量的方法。方法:采用反相高效液相色谱法。色谱柱为Waters C18柱,流动相为乙腈-水(34:66),流速为1.0 mL.min-1,检测波长为290nm,进样量为10μL,柱温为30℃。结果:桂皮醛进样量在0.03942～0.4731μg(r=0.9999)范围内与峰面积积分值线性关系良好;平均加样回收率为98.8%,RSD为0.8%(n=6)。结论:该方法快速简便、准确可靠,重复性好,可用于小建中片中桂皮醛的含量测定。OBJECTIVE： To establish the method for the content determination of cinnamaldehyde in Xiaojianzhong tablet. METHODS： RP-HPLC method was adopted. The determination was performed on Waters C18 column with a mobile phase consist- ed of acetonitrile-water （34：66） at the flow rate of 1.0 mL.min-1. The detection wavelength was set at 290 nm and column temper- ature was 30 %. The injection volume was 10 μL. RESULTS： The linear range of cinnamaldehyde was 0.039 42-0.473 1 μg（r= 0.999 9）with an average recovery of 98.8% （RSD=0.8%, n=6）. CONCLUSION： The established method is simple, convenient, accurate, reliable and reproducible. It can be used for quality control of cinnamaldehyde in Xiaojianzhong tablet.

关 键 词：小建中片 桂皮醛 反相高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]