HPLC法测定养阴清肺丸中芍药苷的含量  

Content Determination of Paeoniflorin in Yangyin Qingfei Pills by HPLC

作　　者：戚宝婵[1] 康强[1] 李子[1] 贾天柱[2] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023 [2]辽宁中医药大学,辽宁大连l16600

出　　处：《中国药房》2012年第32期3053-3055,共3页China Pharmacy

摘　　要：目的:建立测定养阴清肺丸中芍药苷含量的方法,考察不同企业养阴清肺丸中芍药苷含量的差异。方法:采用高效液相色谱法。色谱柱为Agilent C18柱,柱温为40℃,流动相为乙腈-磷酸三乙胺溶液(15∶85),流速为0.8mL.min-1,检测波长为230nm,进样量为10μL。结果:芍药苷进样量在0.094 57～1.4186μg范围内与峰面积积分值呈良好的线性关系(r=0.999 2);平均加样回收率为91.72%,RSD=2.7%(n=6)。结论:该方法简便、准确、灵敏度高、重复性好,可用于养阴清肺丸的质量控制。OBJECTIVE： To establish a method for the content determination of paeoniflorin in Yangyin qingfei pills, and to study the content differences of paeoniflorin in Yangyin qingfei pills from different enterprises. METHODS： The determination was performed on the Agilent C18 column at 40 ℃ with mobile phase consisted of acetonitrile-phosphoric acid triethylamine （15：85） at the flow rate of 0.8 mL.min-1. The detection wavelength was set at 230 nm and injection volume was 10 μL. RESULTS： The lin- ear range of paeoniflorin was 0.094 57-1.418 6μg （r=0.999 2）with an average recovery of 91.72% （RSD=2.7%, n=6）. CON- CLUSION： The method is simple, accurate, sensitive and reproducibility for the quality control of Yangyin qingfei pills.

关 键 词：养阴清肺丸 高效液相色谱法 芍药苷 含量测定 

分 类 号：R917[医药卫生—药物分析学]