HPLC法测定金嗓清音丸中丹皮酚和芍药苷的含量  

Determination of Paeonolum and Paeoniflorin in Jinsangqingyin Pills by HPLC

作　　者：何绍萍 唐丽丽 陈华龙 

机构地区：[1]韶关市食品药品检验所,广东韶关512028

出　　处：《解放军药学学报》2012年第4期336-338,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立HPLC法测定金嗓清音丸中丹皮酚和芍药苷的含量测定方法。方法采用HPLC法,以依利特Hypersil ODS2(5μm,4.6 mm×200 mm)为色谱柱,丹皮酚以甲醇-水(55∶45)为流动相,检测波长为274 nm。芍药苷以乙腈-0.033 mol·L-1磷酸二氢钾溶液(15∶85)为流动相,检测波长为230 nm。流速均为1.0 ml·min-1。结果丹皮酚的线性范围为0.0427～0.6835μg(r=0.9997),平均回收率为98.7%,RSD为0.73%(n=6);芍药苷的线性范围为0.2084～3.3344μg(r=0.9998),平均回收率为98.6%,RSD为0.96%(n=6)。结论此方法操作简便准确,重复性好,可用于该制剂的质量控制。Objective To establish a method for determination of paeonolum and paeoniflorin in Jinsangqingyin pills by HPLC.Methods HPLC was applied on a Hypersil ODS2（5 μm,4.6 mm×200 mm） column.The mobile phase for paeonolum consisted of methanol and water（55∶ 45） at a detection wavelength of 274 nm.The flow rate was 1.0 ml · min-1.The mobile phase for paeoniflorin consisted of acetonitrile and 0.033 mol · L-1 potassium dihydrogen phosphate solution（15∶ 85） at a detection wavelength of 230 nm and a flow rate of 1 ml · min-1.Results The linear range of paeonolumand paeoniflorin was 0.0427-0.6835 μg（r=0.9997）and 0.2084-3.3344 μg（r=0.9998）.The average recovery of paeonolumand paeoniflorin was 98.7% and 98.6%.RSD was 0.73% and 0.96%.Conclusion This method is simple,accurate,reproducible and suitable for quality control of this drug.

关 键 词：金嗓清音丸 丹皮酚 芍药苷 HPLC 

分 类 号：R927.2[医药卫生—药学]