慢性坐骨神经缩窄损伤导致大鼠背根神经节内质网应激反应  

SCIATIC NERVE CHRONIC CONSTRICTION INJURY INCREASES ENDOPLASMIC RETICULUM STRESS IN DORSAL ROOT GANGLION IN RATS

作　　者：赵序利[1] 罗剑刚[1] 张丽[2] 李慧[1] 丛木林[1] 林小雯[1] 宋文阁[1] 郑宏[3] 傅志俭[1] 

机构地区：[1]山东大学附属省立医院疼痛科,济南250021 [2]北京友谊医院麻醉科,北京100050 [3]新疆医科大学第一附属医院麻醉科,乌鲁木齐830054

出　　处：《中国疼痛医学杂志》2012年第8期495-498,共4页Chinese Journal of Pain Medicine

基　　金：山东省自然科学基金项目资助(ZR2009CQ016)

摘　　要：目的:研究坐骨神经慢性缩窄(chronic constriction injury,CCI)引起大鼠背根神经节内质网应激反应。方法:56只SD雄性大鼠随机分为两组:假手术组(sham组)和手术组(CCI组)(n=28)。手术前、术后1天、4天、7天、14天、21天和28天测定动物机械痛敏和热痛敏,背根神经节GRP78葡萄糖调节蛋白78(glucose-regulated protein 78,GRP78,内质网应激反应的标志蛋白)的含量。结果:与假手术组相比,CCI组的机械痛阈和热痛阈在术后明显下降,背根神经节GRP78蛋白表达在第1天开始升高,第7天达到高峰。结论:坐骨神经慢性缩窄模型可以激活大鼠背根神经节GRP78蛋白表达和内质网应激反应,可能与神经病理性疼痛的形成有关。Objective： To observe the change of endoplastic reticulum stress （ERS） in dorsal root ganglion of neuropathic pain rats caused by sciatic nerve chronic constriction injury （CCI）. Methods： 56 adult SD rats were randomly and evenly divided into two groups, sham group （n = 28） and CCI group （n = 28）. Thermal and mechanical nociceptive thresholds were assessed with paw withdrawal latency （PWL） to radiant heat and paw withdrawl thresholds （PWTs） to yon Frey filament. The glucose-regulated protein 78 （GRP78, a marker protein of ERS） was measured with Western Blot. Results： Compare with sham group, the PWT and PWL decreased significantly from day 1 to day 28 in CCI group （P 〈 0.001）, and reached the maximum level in day 7. Sciatic nerve CCI also significantly increased GRP78 protein expression beginning at day 1 and reaching a maximum at day 7 after sciatic nerve injury. Conclusion： The ERS in the dorsal root ganglion was increased after sciatic nerve CCI, which may contribute to the formation of neuropathic pain.

关 键 词：内质网应激 慢性缩窄性损伤 GRP78 神经病理性疼痛 

分 类 号：R651.3[医药卫生—外科学]