神经内分泌细胞浸银染色方法的探讨与应用  

Application and exploration of silver impregnation method used in the staining of neuroendocrine cells

作　　者：王新亭[1] 

机构地区：[1]徐州医学院组织胚胎学教研室,江苏徐州221002

出　　处：《徐州医学院学报》2000年第2期96-99,共4页Acta Academiae Medicinae Xuzhou

摘　　要：目的　探讨用以研究神经内分泌细胞的浸银染色方法。方法　采用Grimelius还原法取代Linder还原法 ,对Linder浸银法加以改良 ,用以显示神经内分泌细胞。应用浸银改良法对固定液及浸银温度、时间等影响因素作了实验研究以及与Grimelius嗜银法和M -H亲银法作了比较研究。结果　浸银改良法显示神经内分泌细胞的形态和嗜银颗粒清晰可辨。经Bouin液固定和浸银反应 40℃ ,10～ 2 5min ,染色效果最佳。与Grimelius嗜银法相比所显示的神经内分泌细胞数有显著性差异 (P <0 .0 1)。结论　浸银改良法用以研究神经内分泌细胞 ,具有特异性和嗜银性强、结果可靠、简便快捷等优点。Objective To improve the silver impregnation method used in the study of neuroendocrine cells.?Methods In order to demonstrate neuroendocrine cells, Linder silver impregnation method was improved by substituting Grimelius development method for Linder development method. The improved method was studied in respects of 5 fixation fluids and time and temperature of silver impregnation.The results obtained were compared with those by Grimelius argyrophil method and M-H argentaffin method.?Results The shape and argyrophil granules of neuroendocrine cells were clearly showed by the improved method, the staining effect was best obtained by Bouin fluid fixation and silver impregnation at 40 ℃ for 10-25 min. The number of the neuroendocrine cells showed by the improved method increased markedly in contrast to that revealed by Grimelius method (P<0.01).?Conclusion High specificity and argyrophilia, reliability of the results and the simple rapid procedures are the merits of the improved method for the study of neuroendocrine cells.

关 键 词：神经内分泌细胞 浸银染色 改良法 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] R335[医药卫生—基础医学]