地塞米松预防新生大鼠缺氧缺血性脑病的作用  

Dexamethasone prevents neonatal hypoxic- ischemic encephalopathy

作　　者：蒋予[1] 李瑞林[1] 黄绍平[1] 吴建维[1] 

机构地区：[1]西安医科大学第二临床医学院儿科,710004

出　　处：《广东医学》2000年第4期291-292,共2页Guangdong Medical Journal

摘　　要：目的研究地塞米松（Ｄｅｘ）预处理对新生大鼠缺氧缺血性脑病（ＨＩＥ）的预防作用及其机理。方法 ４ ｄ龄ＳＤ大鼠随机分为 ３组，分别给予生理盐水［１０ ｍｌ（ｋｇ·ｄ）］、低剂量 Ｄｅｘ［０．１ｍｇ／（ｋｇ·ｄ）］、高剂量 Ｄｅｘ［０．５ ｍｇ／（ｋｇ·ｄ）］预处理４ ｄ，然后结扎左侧颈总动脉，放入 ３７℃恒温有机玻璃缺氧房中，暴露于 ９２％氮气和 ８％氧气的环境中 ３ ｈ。分别于生后１０ ｄ取全脑标本切片作 ＨＥ染色，观察梗死发生率、梗死面积、大脑皮层细胞超微结构变化。结果低剂量 Ｄｅｘ组、高剂量Ｄｅｘ组的梗死发生率和梗死面积均低于生理盐水组（Ｐ＜ ０．００１），Ｄｅｘ组间差异无显著性（ Ｐ＞ ０．０５）。 Ｄｅｘ可明显改善大脑神经细胞的超微病理学变化。结论地塞米松预处理对新生大鼠缺氧缺血性脑损害具有预防作用，其神经保护机制可能是通过延缓神经发育成熟实现的。Objective To investigate the effects of dexamethasone pretreatment on neonatal HIE. Method All 4 - d - old Sprague - Dawley rat pups (n = 60) were randomized to one of three treatment groups and beated with either 0. 1 mg/kg saline or 0. 1 or 0.5 mg/kg dexamethasone for 4 consecutive days. On the 7th postnatal day, arter ligation of the left common carotid artery, the rats were exposed to 8% oxygen - 92% nitrogen at 37℃ for 3 hours and decapitated on the 10th postnatal day. The baains were subsequently fixed by perfusion and stained with hematoxylin - eosin. The area and incidence of infaretion, the supermicro - construction of neuronal cells in cortex were observed. Rasults The infarction was smaller and the area incidence of infaretion were lower in both Dex groups than in con- trols (P < 0.00l). The rats in both Dex groups showed milder damage of the supermicro - construction of neuronal cells in cortex. Con- clusion Dexamethasone pretreatment prevents HIE through suppression of neuronal maturation in neonatal rat.

关 键 词：地塞米松 缺氧缺血性脑病 新生大鼠 预防 

分 类 号：R722.120.1[医药卫生—儿科] R977.11[医药卫生—临床医学]