基因学快速检测脑脊液常见细菌感染的方法  

Rapid detection of common bacterial infections of cerebrospinal fluid by genetics approach

作　　者：管义祥[1] 沈剑虹[2] 居兴云[1] 吴德模[1] 丁锦荣[1] 仲悦萍[2] 张鸣飞[3] 张春秀[4] 

机构地区：[1]南通大学附属海安人民医院神经外科,江苏省南通市226600 [2]南通大学附属医院神经外科 [3]江苏省如东县人民医院 [4]生物芯片上海国家工程研究中心

出　　处：《中华神经科杂志》2012年第8期586-589,共4页Chinese Journal of Neurology

基　　金：南通市社会发展科技计划资助项目（SL0935）

摘　　要：目的探讨基因芯片技术对于颅内感染致病菌快速检测的应用价值。方法选择4种常见致病菌以及6种常见耐药基因的特异DNA序列设计相应的引物与探针，对30例颅内感染患者的脑脊液标本（脑脊液培养阳性12例，阴性18例）进行多重PCR扩增、基因芯片检测，进行菌种鉴定和耐药基因检测，与脑脊液细菌培养及药敏检测结果进行比较。结果15例标本鉴定出菌种并检出耐药基因；8例未鉴定出菌种但检出细菌及耐药基因；7例未检出细菌及耐药基因。结论基因芯片可以相对灵敏、快速地用于颅内感染患者脑脊液中致病菌检测。Objective To assess gene chip application value in detecting pathogenic bacteria in intracranial infection cases. Methods Primers and probes aiming at the specific DNA sequences of 4 kinds of common pathogenic bacteria and 6 kinds of common drug resistance genes （DRGs） were designed and used to identify the bacteria and DRGs among 30 cerebrospinal fluid （CSF） specimens （ 12 positive, 18 negative in CSF culture） from patients with intracranial infection using multiplex polymerase chain reaction （mPCR） and gene chip. The results of gene detection were compared with those of CSF culture and drug sensitivity testing. Results Bacteria were identified and DRGs were detected in 15 specimens; DRGs and 16S gene were detected in 8 specimens; neither bacterium nor DRG was detected in 7 specimens. Conclusion Gene chip technique is characterized by its relative sensitivity and rapidity of detecting the pathogenic bacteria in CSF of intracranial infection cases.

关 键 词：中枢神经系统细菌感染 脑脊髓液 寡核苷酸序列分析 多重聚合酶链反应 抗药性 细菌 

分 类 号：R446.5[医药卫生—诊断学]