铜锌超氧化物歧化酶与色氨酸钴(Ⅱ)的相互作用  

Study on the Interaction of Copper-Zinc Superoxide Dismutase with Coba lt(Ⅱ)-Tryptophane by Spectral Analysis

作　　者：郑学仿[1] 胡皆汉[2] 王静云[1] 盖宏伟[1] 安利佳[1] 

机构地区：[1]大连理工大学化工学院生物工程系 [2]中国科学院大连化学物理研究所,116023

出　　处：《光谱学与光谱分析》2000年第3期305-307,共3页Spectroscopy and Spectral Analysis

基　　金：辽宁省科委自然科学基金资助项目!(972 0 6 7)

摘　　要：采用 VIS、ICP及酶活性测定等方法 ,研究铜锌超氧歧化酶 (Cu2 Zn2 SOD)与色氨酸钴 ( ) (Co(Trp) n )的直接相互作用以及外加色氨酸钴 ( )的量、溶液 p H值对此类相互作用的影响。结果发现 :在水溶液中原酶活性中心处的 Zn( )离子可被外加的色氨酸钴 ( )部分诱导、交换出来 ,而溶液中外加的 Co(Trp) n 中的 Co( )进入酶的活性中心 ,形成“Co- SOD”酶衍生物 ,并相应影响了酶的催化活性。与此同时 ,外加色氨酸钴 ( )的量、溶液 p H值对此类相互作用有重要的影响。The effect of changing the amount of external ad ded Co(trp) n and pH values on the interaction of Cu 2Zn 2SOD with org anic metal compound (Cobalt(Ⅱ)-Tryptophane) have been studied by means of ICP ,VIS and measurement of enzyme activity.It has found that in aqueous solution,th ere exists a direct interaction of the metal ions of the active center in the me talloenzyme (Cu 2Zn 2SOD) with external added Co(Trp) n.As a result,par t of the metal ions in metalloenzyme were replaced and the corresponding metall oenzyme derivatives (Co(Ⅱ)-substituted derivatives of SOD) were produced and t he catalytic activity of enzyme were affected.It has also studied the intensity of interactions in different molar ratios (Cobalt(Ⅱ)-Tryptophane) and pH value s and got some results under the effect of corresponding factors.

关 键 词：铜锌超氧歧化酶 色氨酸钴 相互作用 NMR VIS 

分 类 号：Q554.9[生物学—生物化学] O627.8[理学—有机化学]