铜(Ⅱ)-新铜试剂-谷胱甘肽-乙醇体系显色反应研究  

Stydy on Color Reaction of Copper(Ⅱ)-Neocuprine-Glutathione-Alcohol System

作　　者：郭黎平[1] 刘国良[1] 张卓勇[1] 刘思东[1] 王丽颖[2] 

机构地区：[1]东北师范大学化学学院,长春130024 [2]白求恩医科大学基础医学院,长春130021

出　　处：《光谱学与光谱分析》2000年第3期412-414,共3页Spectroscopy and Spectral Analysis

基　　金：吉林省卫生厅科研基金资助项目

摘　　要：本文研究了铜 ( ) -新铜试剂 -谷胱甘肽 -乙醇体系的显色反应 ,探讨了反应条件。试验中发现乙醇可以使吸光度增加 13.8% ,提高了显色反应的灵敏度。该方法用于测定大豆提取液中谷胱甘肽的含量 ,取得了满意结果。The colour reaction of glutathione with copper ( Ⅱ)-neocuproine -alcohol was investigated in the present paper.The absorbance increased distinctly by about 13.8% when alcohol was added to the system.The ma ximum absorption wavelength λ max of [Cu(neocuproine) 2]- + was at 456 nm and the apparent molar absorptivity was 1.2×10-4 L·mol- -1·cm- -1 in the selected system.Linear dynamic relationship was obta ined in the concentration range of 2—24 μg·mL- -1 for glutathione.Th e regression equation was as following:A=0.010 4+0.038 5c.The correlat ion coefficients was 0.999 8.The recoveries obtained were between 98.5% and 99 .9%.The relative standard deviations were less than 0.9% for all analyses.This method was used to determine glutathione in soybean extracts by the electromagn etic field technique and satisfactory results were obtained.

关 键 词：新铜试剂 谷胱甘肽 光度法 铜 乙醇 显色反应 

分 类 号：Q516.03[生物学—生物化学] O657.32[理学—分析化学]