Ca^(2+)和Sr^(2+)联合处理对油菜抗氧化酶活性的影响  

Effects of Combined Treatment of Ca^(2+) and Sr^(2+) on Antioxidant Enzymes Activities of Oilseed Rape Seedlings

作　　者：陈梅[1] 敖嘉[1] 唐运来[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010

出　　处：《安徽农业科学》2012年第25期12345-12348,共4页Journal of Anhui Agricultural Sciences

基　　金：四川省科技厅应用基础研究项目(2009JY0144);农业部核农学重点开放实验室基金项目(KYJD09017)

摘　　要：[目的]为了了解环境介质中同时存在Ca2+和Sr2+2种离子时油菜幼苗的抗氧化酶活性的变化。[方法]在石英砂和Hoagland营养液培养体系中,用浓度为0、10、20和40 mmol/L SrCl2及浓度为0、5和10 mmol/L Ca(NO3)2处理3叶龄油菜幼苗。在处理0、7、14和21 d后,分别取样测定POD、CAT和SOD的活性。[结果]在相同的Sr2+浓度下,随着Ca2+浓度的增加,POD活性增加,Ca2+明显缓解Sr2+对油菜幼苗的毒害作用。[结论]该研究从抗氧化角度初步阐明油菜具有较高富集Sr2+能力的机理,为利用油菜对锶污染环境进行植物修复提供理论依据。[Objective] The research aimed to investigate the effects of combined treatment of Sr2＋ and Ca2＋ on antioxidant enzymes activities of oilseed rape seedlings.[Method] Three-leaf stage oilseed rape seedlings（Brassica napus L.cv.Mianyou No.15） were raised in quartz-hydroponic culture system,and a solution containing 0,10,20 or 40 mmol/L SrCl2,and 0,5 or 10 mmol/L Ca（NO3）2 was applied.Three antioxidant enzymes（POD,CAT and SOD） activities were determined after 0,7,14 and 21 d of the combined treatment of Sr2＋ and Ca2＋.[Result] POD,CAT and SOD activities were increased gradually with the increase of Ca2＋ concentration.Ca2＋ could alleviate the deleterious effect induced by Sr2＋ in oilseed rape seedlings.[Conclusion]The strontium accumulation capability of oilseed rape was explained from the perspective of antioxidant rapeseed,providing a theoretical basis for the use of oilseed rape phytoremediation of strontium to cleanup the polluted environments.

关 键 词：油菜 Ca2+和Sr2+ 抗氧化酶活性 植物修复 

分 类 号：S634.5[农业科学—蔬菜学]