玉米重组自交系苗期耐盐相关性状QTL的初步定位  

Mapping of QTL Associated with Salt Tolerance in Maize Inbred Line during Seedling Stage

作　　者：王士磊[1,2] 高树仁[1] 王振华[3] 郎淑平[2] 王敬红[4] 

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163319 [2]嘉兴市农业科学研究院,浙江嘉兴314024 [3]农业部寒地作物生理生态重点开放实验室,黑龙江哈尔滨150030 [4]浙江工业大学生物与环境学院,浙江杭州310014

出　　处：《安徽农业科学》2012年第25期12363-12366,共4页Journal of Anhui Agricultural Sciences

基　　金：农业部寒地作物生理生态重点开放实验室课题;黑龙江省教育厅科研项目(11521196)

摘　　要：[目的]对玉米重组自交系苗期耐盐相关性状进行QTL定位。[方法]以玉米黄早四与Mo17杂交的RIL-F7代171份材料为作图群体,构建其玉米的分子标记遗传图谱,并在此基础上开展对玉米耐盐相差性状的QTL分析。[结果]构建了一张包含81个SSR位点,覆盖了玉米基因组10条染色体,共1 428.3 cM,标记间平均间距为17.63 cM的分子遗传连锁图谱;共检测到6个QTL,分别位于第1、5、6号染色体上。[结论]该研究对深入认识玉米耐盐遗传机理,了解控制玉米耐盐性的基因数目及其在染色体上的位置以及对耐盐性的遗传效应,进行玉米耐盐性育种的分子标记辅助选择和基因克隆均具有极其重要意义。[Objective] This study aimed to map quantitative tarit loci（QTLs） associated with salt tolerance of maize inbred lines during seedling stage.[Method] The recombinant inbred lines（RIL） F7 including 171 plants were developed by single seed descent procedure from a combination,Huangzaosi×Mo17,and used to map QTLs associated with salt tolerance,based on the constructed genetic map of SSR markers.[Result]A linkage map consisting of 81 SSR markers loci from 10 chromosomes（1 428.3 cM in total length,with an average distance of 17.63 cM between two neighbouring loci） was constructed.Six QTLs associated significantly with salt tolerance were detected at chromosomes 1,5 and 6.[Conclusion] This study is extremely significant for better understanding salt tolerance-related genes,the genes’ location and cloning,salt tolerance mechanism and the marker-assisted selection of salt tolerant maize.

关 键 词：玉米 耐盐性 SSR分子标记 QTL 

分 类 号：S513[农业科学—作物学]