苗药双肾草多糖含量测定及其免疫调节作用研究  

Determination of Polysaccharides in Herminium bulleyi and Its Immunoloregulation Effects

作　　者：刘刚[1] 刘育辰[1] 邱德文[1] 王振宇[2] 邱明华[3] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]昆明医学院,云南昆明650031 [3]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650204

出　　处：《安徽农业科学》2012年第25期12459-12461,共3页Journal of Anhui Agricultural Sciences

基　　金：中国科学院"科技之黔工程"项目(黔-01-2005-01)

摘　　要：[目的]测定苗药双肾草中多糖的含量并研究其免疫调节作用。[方法]以葡萄糖为对照品,采用苯酚-硫酸法测定双肾草多糖的含量,并研究双肾草多糖对小鼠的免疫调节作用。[结果]双肾草多糖含量为58.5%;双肾草多糖可明显增加小鼠的脾脏重量,促进抗体的产生,对正常小鼠体液免疫具有一定的增强作用。[结论]双肾草多糖对机体的的特异性免疫功能有一定的促进作用。[Objective] To detect the polysaccharides content in Herminium bulleyi（Rolfe） Tang et Wang and its immunomodulatory effects.[Method] With glucose as the control,polysaccharides content in H.bulleyi was detected by phenol-sulphuric acid method.And the immunomodulatory effects of H.bulleyi polysaccharides on mice were researched.[Conclusion] Content of H.bulleyi polysaccharides was 58.5%,which could significantly enhance the spleen weight of mice,promote the development of antibody,and enhance the humoral immunity of mice.[Conclusion] H.bulleyi polysaccharides had certain promotion effects on the specific immunity of organism.

关 键 词：双肾草 多糖 含量测定 免疫调节 

分 类 号：S567[农业科学—中草药栽培]