鸡恒定链活性片段和NDVF蛋白表位嵌合体的构建与鉴定  

Construction and Identification of Chimera Containing Active Segments of Chicken Invariant Chain and F Protein Epitope of NDV

作　　者：倪庆胜[1] 陈芳芳[1] 余为一[1] 

机构地区：[1]安徽农业大学安徽省人兽共患病重点实验室,安徽合肥230036

出　　处：《安徽农业科学》2012年第25期12556-12557,12574,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31172306)

摘　　要：[目的]为研究基于恒定链(Ii)分子片段的免疫载体提供试验材料。[方法]利用鸡恒定链的部分有免疫活性的片段作为免疫载体结构,构建含新城疫病毒F蛋白抗原片段的嵌合体。[结果]成功构建了Ii-key/F306、Ii-key/F306/AP、Cyt/Ii-key/F306和Cyt/Ii-key/F306/AP 4个嵌合体。重组质粒能被诱导表达相应蛋白分子,而且通过纯化获得95%高纯度。[结论]该试验中嵌合体的成功制备为动物免疫试验奠定了基础。[Objective] The research aimed to provide experimental materials for studying the immune carrier based on the molecular segments of invariant chain（Ii）.[Method] Using partial segments with immune activity of chicken invariant chain as the structure of immune carrier,the chimera containing the segments of F protein antigen of newcastle disease virus（NDV） was constructed.[Result] Four chimeras were successfully constructed,including Ii-key/F306,Ii-key/F306/AP,Cyt/Ii-key/F306 and Cyt/Ii-key/F306/AP.The recombinant plasmids could be induced to express the related protein molecules.And these protein molecules could be purified to 95% in purity.[Conclusion] The preparation of these chimeras laid the foundation for performing vaccination test on animals.

关 键 词：鸡恒定链 新城疫病毒 融合蛋白 嵌合体 原核表达 纯化 

分 类 号：S855.3[农业科学—临床兽医学]