转基因抗虫棉Bt毒蛋白含量时空变化及土壤降解研究  

Study on Spatial-temporal Dynamics of Bt Toxic Protein Expression in Insect-resistant Transgenic Cotton and Its Degradation in Soil

作　　者：张益文[1] 王连荣[2] 张连成 张军[1] 籍新波[1] 王进茂[1] 

机构地区：[1]河北农业大学林学院,河北保定071001 [2]河北北方学院园艺系,河北张家口075000 [3]天津滨海新区塘沽林业工作站,天津300450

出　　处：《安徽农业科学》2012年第25期12587-12590,共4页Journal of Anhui Agricultural Sciences

基　　金：转基因生物新品种培育重大专项(2009ZX08011-027B);国家自然科学基金资助项目(30872050)

摘　　要：[目的]研究转基因抗虫棉Bt毒蛋白含量的时空变化及其土壤降解。[方法]采用ELISA法(酶链免疫法)研究和分析了转Bt-cry1Ac基因抗虫棉的根、茎和叶片组织在不同发育时期毒蛋白的含量变化及转Bt基因抗虫棉(GK45)和非转基因棉花(新陆早36号)在根际土壤、表层土壤和后茬种植区土壤中Btcry1Ac毒蛋白的年平均含量变化。[结果]BtCry1Ac毒蛋白含量在抗虫棉生长过程中均呈动态下降趋势,而根中下降的速率最快,茎和叶片次之;棉花种植区土壤表层中均检测到Btcry1Ac毒蛋白,且后茬种植区中表层毒蛋白的含量增加,而根际土中含量极低。[结论]Btcry1Ac毒蛋白的含量检测为种植转基因作物的风险评价及转基因作物的土壤生态系统安全性评价提供了科学依据。[Objective] This study aimed to investigate the spatial-temporal dynamics of Bt toxic protein expression in insect-resistant transgenic cotton and its degradation in soil.[Method] Btcry1Ac toxic protein expression in the roots,stems and leaves of transgenic cotton Guoshen GK45 at different developmental stages and the annual average content of BtCry1Ac toxin protein in the topsoil,rhizosphere soil and later crop-growing area were explored and analyzed by using enzyme linked immuno sorbed assay（ELISA）.[Result] The content of exogenous BtCry1Ac toxin protein decreased during the growth process of insect-resistant transgenic cotton,to be specific,the content of BtCry1Ac toxin protein in cotton stems and leaves decreased more slowly and always maintained a high level,while that in roots decreased rapidly and reached a minimum level to the later plant growth and development stage.BtCry1Ac toxin protein was detected in topsoil of both non-transgenic and transgenic cotton-growing areas,and the content of BtCry1Ac toxin protein increased in topsoil of later crop-growing area,which was very low in rhizosphere soil.[Conclusion] Determination of Btcry1Ac toxic protein provides scientific basis for the risk assessment of the cultivation of genetically modified crops and the safety evaluation of soil ecosystem.

关 键 词：转基因棉花 BT毒蛋白 土壤微生物 安全性评价 

分 类 号：S722[农业科学—林木遗传育种]