黄芪多糖提高HL-60细胞对NK细胞杀伤活性的敏感性及其机制  

Effect of Astragalus Polysaccharide on Sensitivity of Leukemic Cell Line HL-60 to NK Cell Cytotoxicity and Its Mechanism

作　　者：曾鹏云[1] 邓黎黎[1] 岳玲玲[1] 张连生[1] 

机构地区：[1]兰州大学第二附属医院血液科,甘肃兰州730000

出　　处：《中国实验血液学杂志》2012年第4期880-883,共4页Journal of Experimental Hematology

摘　　要：本研究探讨黄芪多糖处理后HL-60细胞对NK细胞敏感性的变化,并初步探讨其机制。用LDH释放法检测NK细胞对黄芪多糖处理48 h后HL-60细胞的杀伤活性;用RT-PCR、流式细胞术分别检测15 mg/ml黄芪多糖处理48h后HL-60细胞MHCⅠ类相关蛋白酶A(MICA)基因和蛋白水平的表达。结果表明,黄芪多糖作用48 h后,HL-60细胞在不同效靶比(5∶1、10∶1、20∶1)时对NK细胞杀伤敏感性显著增加,差异有统计学意义(P<0.05),HL-60细胞MI-CA的表达在基因和蛋白水平均有显著增加,差异有统计学意义(P<0.05)。结论:黄芪多糖能提高HL-60细胞对NK细胞杀伤活性的敏感性,其机制可能与黄芪多糖提高HL-60细胞表面MICA的表达有关。The objective of this study was to explore the effect of astragalus polysaccharide （ APS ） on sensitivity of leukemic cell line HL-60 to NK cell cytotoxicity and its mechanism. The cytotoxicities of NK cells against HL-60 ceils were analyzed by LDH releasing assay at different effect-to-target cell ratios （E： T） before and after treated with APS. The gene expression of MHC class I chain-related（MICA） in HL-60 ceils before and after APS treatment was assayed with RT-PCR. Protein expression of MICA in HL-60 cells was assayed by flow cytometery before and after treated by APS. The results showed that after treated with APS 15 mg/ml for 48 h, the cytotoxicities of NK cells against HL-60 cells enhanced at different effect-to-target （ P 〈 0.05 ）, and the gene and protein expressions in MICA of HL-60 cells were up-regulated （ P 〈 0.05 ）. It is concluded that the APS can obviously up-regulate the expression of MICA in HL450 ceils, thus enhance sensitivity of HL-60 cells to cytotoxicity of NK ceils.

关 键 词：黄芪多糖 白血病HL-60细胞 MICA NK细胞 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]