GCC盒和JERE盒介导的信号途径在水稻应答逆境中的作用  

Functional Analysis on GCC Box and JERE Box Mediating Signal Pathway in Rice Response to Stress

作　　者：牟少亮[1] 黄定全[1] 刘志钦[1] 何水林[1] 

机构地区：[1]福建农林大学生命科学学院,福州350002

出　　处：《应用与环境生物学报》2012年第4期586-590,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.30971718);国家转基因生物新品种培育重大专项(No.2009ZX08001-015B);福建省自然科学基金项目(No.2007J0046)资助~~

摘　　要：植物应答逆境的防御反应在很大程度是在基因转录水平调节的,其中转录因子与目标基因启动子上顺式作用元件的识别和结合起着关键的调节作用.本研究将从双子叶植物中发现的GCC盒(应答乙烯)和JERE盒(应答JA和激发子)与CaMV35S核心启动子融合构建成诱导型启动子,利用GUS报告基因构建其表达载体并进行农杆菌介导的水稻遗传转化.利用T1代株系分析了GCC盒和JERE盒水稻植株对不同逆境胁迫和激素处理的应答.结果表明在转基因植株中它们都具有很低的本底表达.稻瘟病侵染、稻纵卷叶螟取食和机械损伤处理可不同程度提高GUS基因的表达.另外,脱落酸(ABA)、水杨酸(SA)和茉莉酸(JA)也能提高GUS的表达.实验结果暗示JERE和GCC盒介导的信号途径在单子叶和双子叶之间有一定的保守性.Plants respond to stresses are mainly regulated by transcriptional modulation. And it is vital that transcription factors recognize and bind to their corresponding cis-elements in the promoters of target genes. In this study, two synthetic promoters were constructed by combining GCC box （ET responsive） or JERE box （JA and elicitor responsive） with the 35S minimal promoter of cauliflower mosaic virus, respectively. The promoters were further cloned into expression vectors to drive β-glucuronidase （GUS） reporter gene and the vectors were transformed into rice via an Agrobacterium-mediated transformation method. The expression of GUS in T1 transgenic lines under different stimuli was analyzed. The results showed that two synthetic promoters had very low background expression. GUS expression could be improved to varying degree by rice blast infection, Cnaphalocrocis medialis biting and mechanical wounding. Moreover, GUS expression could also be improved by ABA, SA and JA. The results suggested a conservation of GCC and JERE mediating defense signaling pathways between dicotyledon and monocotyledon. Fig 5, Ref 15

关 键 词：GCC盒 JERE盒 信号途径 水稻 作用分析 遗传转化 抗逆反应 

分 类 号：Q943.2[生物学—植物学] S511.034[农业科学—作物学]