一种模拟失重性骨丢失小鼠尾悬吊模型的建立  

Construction of a Modified Mouse Tail-suspension Model for Space Bone Loss

作　　者：林煜[1] 刘宗琳[1] 骞爱荣[1] 商澎[1] 

机构地区：[1]西北工业大学生命学院,西北工业大学特殊环境生物物理学研究所,空间生物实验模拟技术国防重点学科实验室,陕西西安710072

出　　处：《航天医学与医学工程》2012年第4期239-242,共4页Space Medicine & Medical Engineering

基　　金：国家973计划课题(2011CB710903);西工大基础研究基金资助(JC201055)

摘　　要：目的建立一种模拟失重性骨丢失小鼠尾悬吊模型。方法根据Morey-Holton的大鼠尾悬吊模型,加以改进,以小鼠为模式动物,进行了为期4周的小鼠尾部悬吊实验,并对小鼠的股骨进行HE染色,组织形态学观察和力学性能检测。结果在尾悬吊28 d后小鼠体重(32.11±2.79)g较对照组(37.36±2.44)g下降了14%。组织学观察显示,与对照组相比,尾悬吊小鼠股骨皮质骨内未钙化的骨基质增多,骨基质中的骨细胞较幼稚,成骨细胞少,破骨细胞多而大。股骨力学性能结果显示,尾吊小鼠股骨的刚度(P<0.05)和最大载荷(P<0.001)与对照组有明显的下降。结论尾吊28 d后的小鼠股骨发生了骨丢失,该模型可以用于模拟失重生物效应的体内实验研究。Objective To construct a mouse tail-suspension model for space bone loss. Methods Mice were tail-suspended for 4 weeks according to the modified Morey-Holton＇ s rat tail-suspension model. The histology of the femur was observed by the hematoxylin-eosin （ HE ） staining. The mechanical properties of the femur were determined by mechanical testing. Results Body weights of the mice in the tail-suspension （TS） group （32.11 ± 2.79） g decreased by 14% as compared with those of control（Con） group （37.36 ± 2.44） g after 28 d tail suspension. Histological observation showed that, uncalcific matrix was increased and bone cells were infantile in femur of suspension group as compared with control group. Meanwhile, the number of osteoblasts decreased and the number of osteoclasts increased. Mechanical testing showed that the stiffness and the ulti- mate force were significantly declined （P 〈0.05 and P 〈0. 001 ） as compared with those of the control group. Conclusion The findings indicates that 28 d of the tail-suspension can lead to bone loss in mice femur, and this model can be used to study furtherly the biological effects of weightlessness in vivo.

关 键 词：尾吊模型 骨丢失 小鼠模型 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]