亲和毛细管电泳测定孕酮与其单克隆抗体的结合常数  

Determination of the Binding Constant Between Progesterone and Its Monoclonal Antibody Using Affinity Capillary Electrophoresis

作　　者：王清刚[1] 王义明[1] 罗国安[1] 杨树标[2] 

机构地区：[1]清华大学化学系,北京100084 [2]香港大学妇产科系

出　　处：《分析化学》2000年第6期731-734,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：采用亲和毛细管电泳的配体分离模式，以激光诱导荧光作为检测手段，测定了荧光素标记的孕酮与孕酮单克隆抗体之间的结合常数，并研究了温育时间、电泳条件等因素对测定的影响。The binding constant (Kb) between fluorescein isothiocyanate labeled progesterone (FP) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis with laser induced fluorescence (LIF) detection.Free FP and the complexes were separated by capillary electrophoresis in 3 min after 90 min incubaion.The running buffer was 50 mmol/L Tricine with pH 8.4, and separation voltage was 20 kV.The Kb was determined to be 4.4x10' L/mol using Scatchard plotting method.The influences of incubation time and electrophoresis conditions on the determination were also studied.

关 键 词：亲和毛细管电泳 孕酮 结合常数 单克隆抗体 

分 类 号：O657.8[理学—分析化学]