壮药柿叶槲皮素和山柰素含量测定方法的建立  

Establishment of Content Determination of Quercetin and Kaempferol in Zhuang Medicine of Persimmon Leaves

作　　者：覃冬杰[1] 梁永红[1] 黄瑞松 刘华钢[1] 张鹏 

机构地区：[1]广西医科大学药学院,南宁市530021 [2]广西民族医药研究院,南宁市530001

出　　处：《广西医学》2012年第9期1133-1136,共4页Guangxi Medical Journal

基　　金：广西壮药质量标准研究项目(MZY2010074)

摘　　要：目的建立柿叶槲皮素和山柰素含量测定方法。方法对HPLC方法中的色谱条件进行摸索,并采用正交设计法考察供试品溶液的制备条件。结果色谱条件为:Hypersil ODS2 C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.4%磷酸溶液(50∶50),流速1.0 ml/min,检测波长360 nm。样品供试液制备条件为:药材破碎度过60目筛,提取溶剂为甲醇-25%盐酸(4∶1),溶剂加入量10 ml,回流时间1 h。结论建立的HPLC含量测定方法准确可靠,可作为壮药材柿叶中槲皮素和山柰素总量的测定方法。Objective To investigate the method for content determination of quercetin and kaempferol in persimmon leaves. Methods To research the chromatographic conditions of high performance liquid chromatography （ HPLC ）, then the orthogonal design was applied to study the samples＇ preparation conditions. Results Chromatographic condition as follows ： Hypersil ODS2 C18 （4.6 mm × 250 mm ,5μm） ,the mobile phase consisted of methanol - 0.4% phosphoric acid（50 ： 50） with the flow of 1.0 ml/min,and the detection wavelength was 360 nm. The samples＇ preparation conditions as follows ： The fragmentation degree was 60 mesh, extraction solvent was methanol - 25% hydrochloride （4 ： 1 ）, solvent inventory amounted to l0 ml, reflux time was 1 hour. Conclusion The HPLC method we established is accurate and reliable, which can be used for determination of quercetin and kaempferol in Zhuang medicine of persimmon leaves.

关 键 词：柿叶 槲皮素 山柰素 含量测定 HPLC 

分 类 号：R284.2[医药卫生—中药学] R965.2[医药卫生—中医学]