高效液相色谱法测定人血浆中的辛伐他汀  

Determination of Simvastatin in Human Plasma by High Performance Liquid Chromatography

作　　者：谭力[1] 杨丽莉[1] 张昕[1] 袁倚盛[1] 凌树森[1] 

机构地区：[1]南京军区南京总医院临床药理科,江苏南京210002

出　　处：《色谱》2000年第3期232-234,共3页Chinese Journal of Chromatography

摘　　要：建立了高效液相色谱法监测人口服辛伐他汀药物后的血药浓度。血样用环己烷 -二氯甲烷 (体积比为 3.5∶ 1)提取 ,以洛伐他汀为内标 ,在 2 37nm波长下检测 ;色谱柱 :Lichrospher C1 8( 2 0 0 mm× 4 .6mm i.d.,5μm ) ,流动相 :乙腈 -水 (体积比为 70∶ 30 ) ;流速 :1.2 m L/ m in。血药浓度在 0 .2 5～ 50 .0μg/ L范围内与峰面积和内标峰面积的比值之间线性关系良好 ,日内及日间相对标准偏差 ( n =5)分别低于 7.94 %和 8.58% ,回收率高于93.3%。方法灵敏、准确、快速 。Simvastatin is widely used in the treatment of hypercholestero lemia. Recently, it was reported that it is also effective in reducing lethality in coronary heart disease. A simple and sensitive reversed phase liquid chromatographic method has been developed and validated for the analysis of simvastatin in human plasma. Simvastatin and internal standard lovastatin in plasma were extracted with cyclohexane dichloromethane (3.5∶1, V/V ), and then measured by HPLC using a Lichrospher C 18 column as stationary phase and an acetonitrile water (70∶30, V/V ) mixture as mobile phase.The flow rate was 1.2 mL/min. Simvastatin was quantified by UV at 237 nm. The calibration curve showed a good linearity in the mass concentration range of 0.25\|50.0 μg/L. The regression equation is: Y=0.036 4ρ + 0.005 83, r =0.999 8. Intra day and inter day coefficients of variation of assay for simvastatin in plasma <7.94% and <8.58%, respectively. The recoveries of simvastatin were >93.3%. The method has been used to determine simvastatin in plasma from 12 healthy male volunteers.

关 键 词：高效液相色谱法 辛伐他汀 血药浓度 测定 血浆 

分 类 号：R972.6[医药卫生—药品] R969.1[医药卫生—药学]