HPLC测定紫黄凝胶剂中左旋紫草素的含量  

Determination of L-Shikonin in Zihuang Gel by HPLC

作　　者：林雄[1] 严国鸿[1] 潘旭东[1] 黄燕[1] 

机构地区：[1]福建中医药大学附属人民医院,福州350004

出　　处：《海峡药学》2012年第7期52-53,共2页Strait Pharmaceutical Journal

基　　金：福建省卫生厅青年科研课题(项目编号:2010-2-70)

摘　　要：目的建立紫黄凝胶剂中左旋紫草素的含量测定方法。方法采用HPLC法,色谱柱为Acclaim 120 C18（4.6×250mm,5μm）,流动相为甲醇-水-磷酸（78∶22∶0.3,v/v）,检测波长为516nm,柱温为35℃。结果在8.56-42.80μg·mL-1之间,左旋紫草素进样量和峰面积具有良好的线性关系,线性方程为y=0.4088x＋0.033,r=0.9999;测得紫黄凝胶剂中左旋紫草素最低平均含量为0.0615mg·g-1。结论该方法简便可行,重现性好,可作为紫黄凝胶剂的质量控制方法。OBJECtiVE To establish the determination method of L-Shikonin in Zihuang gel. METHODS The column was Acclaim 120 C18（4.6 x250mm, 5μm）, mobile phase was methanol-water-phosphoric acid（78：22 ：0.3, v/v）, the detection wavelength was 516nm, column temperature was 35°C. RESULTS L-shikonin sample volume and the peak area had a good linear relationship in 8.56-42.80μg. mL-1, linear equation was y = 0. 4088x ＋ 0.033, r = 0.9999; the lowest average concentration of L-Shikonin in Zihuang gel was 0.0615mg.g-1 CONCLUSION The method is simple, feasible, and reproducible and can be used as the quality control method for Zihuang gel.

关 键 词：紫黄凝胶剂 HPLC 左旋紫草素 含量测定 

分 类 号：R927.2[医药卫生—药学]