一个Ds插入标签基因OsPI-PLC2的分子鉴定及表达分析  

Characterization and expression analysis of a Ds-tagging line OsPI-PLC2 in rice

作　　者：高志超[1,2] 栾维江[1,2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市细胞遗传与分子调控重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2012年第3期85-90,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市自然科学基金重点资助项目(11JCZDJC17900);天津市教委资助项目(20090609);天津师范大学青年骨干教师学术创新推进计划资助项目(52X09039)

摘　　要：在前期构建的水稻转座子标签插入突变体库中,鉴定了一个没有明显表型变化的Ds转座子标签系,发现Ds标签插入到一个编码磷脂酰肌醇磷脂酶C(PI-PLC)基因的第6个内含子中,分析该基因编码的氨基酸序列发现其含有已知的PI-PLC所具有的典型的X、Y和C2-like保守结构域,与已知的其它植物的PI-PLC蛋白有较高的同源性.另外,利用RT-PCR对该基因进行了表达分析,结果表明该基因在正常条件下表达量很低,但在胁迫条件下(机械损伤、盐、干旱、低温)或一些信号分子(SA、ABA)的诱导下大量表达,表明该基因参与植物对不良环境条件的信号转导途径,对于植物抵御不良环境条件具有重要作用.ADs tagging line OsPI-PLC2 without phenotype change was characterized by rice insertional mutant library. Ds-tag inserted into the sixth intron of OsPI-PLC2 gene which encoded a phosphoinositide-specific phospho-lipase C protein. The sequence analysis showed that OsPI-PLC2 protein contained typical X, Y and C2-1ike domains which were very conservative in known PI-PLC protein and had high homology with known PI-PLC proteins of other plants. Moreover, the expression pattern of OsPI-PLC2 gene was further investigated by using RT-PCR, and it was demonstrated that OsPI-PLC2 gene exhibited weak expression in normal condition hut exhibited strong expres-sion in wound, salt, dry, cold, salicylic acid （SA） and abscisic acid （ABA） induction conditions, suggesting that OsPI-PLC2 gene played an important role in stress signal transduction pathway and resisting harmful environment.

关 键 词：水稻 Ds转座子标签 OsPI-PLC2 表达分析 

分 类 号：Q943[生物学—植物学]