桦褐孔菌菌株遗传多样性的ISSR分析  

Genetic Diversity Among 21 Inonotus obliquus Strains Revealed by ISSR Analysis

作　　者：闫可[1] 尹勇刚[1] 傅常娥[1] 郭晓帆[2] 陈艳秋[1] 

机构地区：[1]长白山生物资源与功能分子教育部重点实验室,延边大学,吉林延吉133002 [2]沈阳农业大学园艺系,沈阳东陵110000

出　　处：《食用菌学报》2012年第2期26-30,共5页Acta Edulis Fungi

基　　金：国家自然基金项目(编号:31160408)的部分研究内容

摘　　要：应用ISSR(inter-simple sequence repeat)分子标记技术对21株不同来源的桦褐孔菌(Inonotusobliquus)菌株进行DNA指纹图谱分析,结果表明:从60条引物中筛选出11条ISSR引物对桦褐孔菌基因组DNA进行了ISSR-PCR扩增,共扩增出DNA谱带183条,其中多态性谱带169条,多态位点百分率为92.3%,遗传相似系数变化范围为0.605～0.880。在遗传相似系数为0.763时,可将21个桦褐孔菌菌株划为6大类群,菌株聚类结果与菌株的地理分布有关。ISSR（inter-simple sequence repeat） molecular markers were used to differentiate 21 Inonotus obliquus wild-type strains originating from different locations worldwide.A total of 183 bands were amplified using 11 ISSR primers（selected from 60 test primers）,of which 169 bands were polymorphic.ISSR similarity coefficients ranged from 0.605-0.880,and a dendrogram based on UPGMA（unweighted pair group method with arithmetic average） cluster analysis revealed that the 21 strains clustered into six groups at a similarity coefficient of 0.763.Cluster analysis further demonstrated that the genetic distance between the different strains was highly correlated to the geographical origin.

关 键 词：桦褐孔菌 ISSR 遗传多样性 

分 类 号：S567.39[农业科学—中草药栽培]