野生硫磺菌分离株ITS序列分析  

ITS Sequence Analysis of Strain ts Isolated from a Fruit Body of Laetiporus sulphureus Growing on Tai Mountain

作　　者：兰玉菲[1] 王庆武[1] 安秀荣[1] 王明才[1] 

机构地区：[1]泰安市农业科学研究院食用菌研究所,山东泰安271000

出　　处：《食用菌学报》2012年第2期31-34,共4页Acta Edulis Fungi

基　　金：山东省中青年科学家科研奖励基金(编号:BS2011NY021);泰安市科技发展计划项目(编号:20113008)的部分研究内容

摘　　要：采用组织分离的方法从野生硫磺菌(Laetiporus sulphureus)子实体上分离得到菌株ts,以其基因组为模板扩增获得该菌株的ITS序列,序列分析结果表明:该菌株与GenBank中Laetiporus sulphureus var.sulphureus的3个日本分离株聚为一簇,ITS核苷酸一致率为98.31%～99.25%,而与其它22个来自不同国家的硫磺菌属菌株的ITS核苷酸一致率为91.93%～98.12%,依此结果,ts菌株应为L.sulphureus var.sulphureus。Fungal mycelium,isolated from a fruit body of Laetiporus sulphureus growing in the wild on a species of Quercus located on Tai Mountain,was identified as a strain（designated strain ts） of L.sulphureus var.sulphureus based on ITS sequencing.The ITS sequence（deposited in GenBank under the accession number,JF946748） was used along with ITS sequences from other Laetiporus species to construct a phylogenetic tree using the neighbor-joining method.This revealed a high genetic similarity（98.31%-99.25%） between strain ts and three L.sulphureus var.sulphureus stains from Japan,and 91.93%-98.12% similarity with the corresponding sequences of 22 Laetiporus strains originating from different countries.

关 键 词：硫磺菌硫磺原变种 转录间隔区 

分 类 号：S646[农业科学—蔬菜学] S567.39[农业科学—园艺学]