檀香叶中牡荆苷与异牡荆苷的含量测定  

Simultaneous Determination of Vitexin and Isovitexin in Leaves of Santalum Album L.by HPLC

作　　者：闫冲[1] 林励[2] 蔡聪 郑来安 

机构地区：[1]广东医学院药学院,广东东莞523808 [2]广州中医药大学中药学院,广州510006 [3]广东省湛江南药试验场,广东湛江524338

出　　处：《中药新药与临床药理》2012年第4期468-470,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家工信部中药材扶持项目(2009060);教育部博士点基金(20094425110009)

摘　　要：目的建立同时测定檀香叶中牡荆苷与异牡荆苷含量测定方法，为其资源利用等提供依据。方法采用Agilent TC—C18色谱柱（4．6mm×250mm，5μm），流动相为甲醇-0．5％冰醋酸溶液（40：60），检测波长为338nm，柱温为30℃，流速为1mL·min^-1。结果牡荆苷的线性范围为7．625～488．0μg·mL^-1；高、中、低剂量回收率分别为96．38％、97.23％、97．96％，RSD为1．29％～1．83％。异牡荆苷的线性范围为8．047～515．01μg·mL^-1，高、中、低剂量回收率分别为97．81％、96．13％、97．98％，RSD为1．31％～1．81％。三批样品中牡荆苷与异牡荆苷的含量为1．13％～1．25％和1．20％～1．46％。结论檀香叶中牡荆苷与异牡荆苷含量较高，所建立的含量测定方法简便、准确，可用于檀香叶的质量控制。Objective To develop a HPLC method for simultaneous determination of vitexin and isovitexin in leaves of Santalum album L. Methods The separation was achieved on a C18 column（4.6 mm × 250 mm, 5 μm. Agilent, USA） with a mobile phase consisting of methanol-0.5 % acetic acid（40 ： 60）. The flow rate was 1 mL·min^-1. The column temperature was maintained at 30 ℃. The detection wavelength was at 338 nm. Results The linear range of vitexin was 7.625-488.0 μg·mL^-1. The recoveries of high-, middle- and low-concentration of vitexin were 96.38 %, 97.23 % and 97.96 % respectively, and the RSD was 1.29 %-1.83 %. The linear range of isovitexin was 8.047-515.0 μg·mL^-1. The recoveries of high-, middle- and low-concentration of isovitexin were 97.81%, 96.13 % and 97.98 % respec- tively, and the RSD was 1.31%-1.81%. The average content of three batches of samples was 1.13 %-1.25 % for vi- texin and 1.20 %-1.46 % for isovitexin. Conclusion The method is accurate and repeatable, which can be used for quality evaluation of vitexin and isovitexin of leaves of Santalum album L.

关 键 词：檀香叶 高效液相色谱法 牡荆苷 异牡荆苷 

分 类 号：R284.1[医药卫生—中药学]