利用大白猪×民猪F_2资源群体开展瘦肉量全基因组关联研究  

Genome Wide Association Study for Lean Meat Weight in A Large White × Minzhu Intercross Population

作　　者：程笃学[1] 罗维真[1] 张龙超 颜华[1] 李勇[1] 王立刚[1] 宋欣[2] 马小军[1] 陈少康[3] 刘欣[1] 李稳[1] 梁晶[1] 赵克斌[1] 王立贤[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]四川农业大学动物医学院,雅安625014 [3]中国农业大学动物科技学院,北京100193

出　　处：《农业生物技术学报》2012年第8期858-866,共9页Journal of Agricultural Biotechnology

基　　金：国家转基因生物新品种培育重大专项(No.2011ZX08006-003);国家"十二五"科技支撑计划项目(No.2011BAD28B01);现代农业产业技术体系和中国农业科学院基本科研业务专项(No.2011cj-5)

摘　　要：高密度单核苷酸多态性(SNP)芯片的出现和基因分型技术平台的发展使在猪上开展全基因组关联分析(GWAS)研究成为现实。本研究以大白猪(Sus scrofa)×民猪(S.scrofa)F2设计资源群体为研究对象,采用Illumina公司猪SNP60K分型芯片技术,开展胴体瘦肉量(LMW)GWAS研究,寻找与瘦肉量相关的遗传变异。所有F2代个体在达到(240±7)d日龄时进行屠宰测定。对分型后的355头F2个体,采用基于混合模型及回归的快速全基因组关联及基因组控制法(GRAMMAR-GC)方法进行GWAS分析,结果获得14个在染色体水平与瘦肉量性状显著关联的SNP位点(P<9.63e-06,SSC1;P<2.37e-05,SSC2;P<1.56e-05,SSC14)。其中2个SNP位点ALGA0010777和ALGA0010788分别位于1号染色体上285030256和285276856bp处;10个SNP位点都位于猪2号染色体末端,可能与已发现的瘦肉量基因突变位点IGF2-intron3-G3072A紧密连锁;2个SNP位点ASGA0065444和ASGA0065455位于14号染色体上99627980和100078535bp处。本研究为猪的瘦肉量性状提供了显著关联SNP位点,预测了新的候选基因。初步阐释了中外猪种瘦肉量性状巨大差异的分子机制,为进一步开展分子育种工作提供了基础研究资料。The high-density single nucleotide polymorphism （SNP） genotyping platform allows genome wideassociation studies （GWAS） in pigs. In this GWAS, 355 pigs from a Large White（Sus scrofa）xMinzhu（S. serofa） intercross population were genotyped using the Illumina Porcine SNP 60K BeadChip, and phenotyped for lean meat weight （LMW） after slaughtered at age of （240~7） d. Association tests between the trait and the SNPs were performed via GWAS using the mixed model and Regression-Genomic Control （GRAMMAR-GC） approach. This GWAS revealed 14 SNPs which showed chromosome-wide significant （P〈9.63e-06, SSC1; P〈2.37e-05, SSC2; P〈l.56e-05, SSC14） association with LWM. On SSC1, two SNPs ALGA0010777 and ALGA0010788, which were located at 285 030 256 and 285 276 856 bp, showed significant association with LWM. Ten significant SNPs were mapped to distal end of SSC2, where the known major gene IGF2 for muscle mass QTL was located. The remainder two SNPs ASGA0065444 and ASGA0065455, which were located at 99 627 980 and 100 078 535 bp on SSC14, were associated with the LWM significantly. The present GWAS results revealed SNPs and candidate genes for LWM in pigs, and the molecular mechanism of LWM difference between Chinese indigenous pig breed and Western commercial pig breed is initially clarified.

关 键 词：全基因组关联研究 瘦肉量 猪 

分 类 号：S831.2[农业科学—畜牧学]