大豆子叶节对卡那霉素的敏感性研究  

Susceptibility of Soybean Cotyledonary Node to Kanamycin

作　　者：张春涛[1] 朱洪德[1] 殷奎德[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319

出　　处：《安徽农业科学》2012年第23期11584-11586,共3页Journal of Anhui Agricultural Sciences

基　　金：国家农业科技成果转化项目(2010GB2B200128)

摘　　要：[目的]研究大豆子叶节对卡那霉素的敏感性,以期为大豆的遗传转化和抗性筛选提供试验依据。[方法]选用3个不同基因型大豆为试验材料,以黄化率和分化率为指标,研究了不同浓度的卡那霉素对大豆子叶节分化和丛生芽生根的影响。[结果]不同基因型大豆对卡那霉素的敏感性不同,子叶节分化的卡那霉素临界筛选浓度为:垦农18为40 mg/L、绥农14和垦农4为60 mg/L;丛生芽生根的卡那霉素临界筛选浓度为:垦农18为15 mg/L、绥农14和垦农4为20 mg/L。[结论]该研究结果为大豆的遗传转化和抗性筛选奠定了基础。[Objective] To study the susceptibility of soybean cotyledonary node to kanamycin,so as to provide experimental basis for the genetic transformation and screening of soybean.[Method] With three different genotypes of soybean as test materials,their yellowing rate and differentiation rate were measured to study the impact of different concentration of kanamycin on the differentiation and shoots growth of soybean cotyledonary node.[Result] Different genotypes of soybean showed different degree of susceptibility to kanamycin,and the critical concentration of kanamycin for the differentiation of cotyledonary was 40 mg/L to Kennong 18,60 mg/L to Suinong 14 and Kennong 4;while the critical concentration of kanamycin for the rooting of shoots was 15 mg/L to Kennong 18,20 mg/L to Suinong 14 and Kennong 4.[Conclusion] The study laid foundation for the genetic transformation and screening of soybean.

关 键 词：大豆 卡那霉素 基因型 敏感性 

分 类 号：S565.1[农业科学—作物学]