不同动物来源肠球菌的分离·鉴定及耐药性研究  

Study on the Isolation,Identification and Drug Resistance of Enterococcus from Different Animal Sources

作　　者：王熙楚[1] 王世旗[1] 王波臻[1] 曹树珠[1] 周霞[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《安徽农业科学》2012年第23期11688-11690,共3页Journal of Anhui Agricultural Sciences

基　　金：人力资源与社会保障部归国留学生科研资助项目(2010-LX007)

摘　　要：[目的]为动物肠球菌感染机制的研究提供理论依据。[方法]采用常规细菌分离方法从不同动物的肛拭样品中分离肠球菌,利用基于肠球菌保守tuf基因的PCR方法对其进行鉴定,同时采用KB纸片法对分离到的肠球菌进行药敏试验。[结果]共分离到177株细菌,经鉴定确定为肠球菌。药敏试验结果表明,鸡源肠球菌对青霉素的耐药率为80%,牛源肠球菌为46.4%;猪源肠球菌对环丙沙星的耐药率为27.3%,羊源肠球菌为2.8%;鸡源肠球菌对高浓度链霉素的耐药率为62.9%,而羊源未出现耐药菌株。[结论]来源于不同动物的正常菌群肠球菌对某些抗生素表现出不同程度的耐药性。[Objective]The research aimed to provide theoretical basis for studying the infection mechanism of enterococcus from animals.[Method]Enterococcus were isolated from anus samples of different animals by using conventional bacterial isolation method.And they were identified by PCR based on conservative tuf gene of enterococcus.Drug test on the isolated enterococcus was conducted by using K-B disk diffusion method.[Result]177 strains of bacteria were isolated and they were identified as enterococcus.The results of drug sensitivity test showed that the drug resistance rate of enterococcus from chicken and cattle to penicillin was 80% and 46.4% respectively.The drug resistance rate of enterococcus from pig and sheep to ciprofloxacin were 27.3% and 2.8% respectively.The drug resistance rate of enterococcus from chicken to streptomycin at high concentrations was 62.9% and enterococcus from sheep showed no drug resistance.[Conclusion]Enterococcus from normal flora of different animal varieties exhibited resistance to antibiotics to different extent.

关 键 词：肠球菌 抗生素 耐药性 

分 类 号：S852.611[农业科学—基础兽医学]