叶绿体基因工程在农业中的应用  

Application of Plastid Transformation in Agriculture

作　　者：罗昌[1,2] 黄丛林[2] 张秀海[2] 吴忠义[2] 

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]北京市农林科学院,北京农业生物技术研究中心,北京100097

出　　处：《安徽农业科学》2012年第24期11968-11971,共4页Journal of Anhui Agricultural Sciences

摘　　要：叶绿体转基因工程与核转基因相比具有明显的优越性,包括:外源基因的高效表达,基因的原核表达形式,无位置效应和基因沉默现象,环境安全性好。目前已经有许多作物品种通过叶绿体基因工程获得了抗除草剂、抗虫、抗病、耐盐耐旱的特性。叶绿体转基因植物是作为生物反应器的理想系统。但是叶绿体转基因也面临着许多挑战,如转化效率低、单子叶植物再生系统的确立与筛选以及叶绿体基因组序列的获得。Chloroplastid transformation has lots of advantages compared with traditional nuclear transformation, including high-efficient expres- sion of exogenous gene, lack of position effect or gene silencing and lower environmental risk. Several crop species have stably integrated transgenes conferring agronomic traits, including herbicide, insect, and disease resistance, drought and salt tolerance. The plant of transplastid is an ideal system for bioreactor. But, this advanced technology is also confronted with a number of challenges, such as low transformation efficiency, ascertain regeneration systems and selection conditions of monocotyledon, sequence chloroplast gcnomes.

关 键 词：叶绿体 质体转基因 核转基因 生物反应器 

分 类 号：S188[农业科学—农业基础科学]