苯酚-硫酸法测定人参茎叶多糖的条件研究  

Determination of Polysaccharides in Ginseng Stem Leaves by Phenol-Sulfuric Acid Method

作　　者：苏晓田[1] 孟凡丽[1] 王雅华[1] 

机构地区：[1]辽宁农业职业技术学院生物技术系,辽宁营口115009

出　　处：《安徽农业科学》2012年第24期12023-12025,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优选苯酚-硫酸法测定人参茎叶多糖含量的因素条件。[方法]采用紫外分光光度法测定人参茎叶多糖的含量,并采用正交试验优选测定的条件。[结果]苯酚-硫酸法测定人参茎叶多糖含量的最佳测定波长为485 nm;苯酚-硫酸法的最佳显色条件为A1B2C1,即:浓度6%苯酚的体积为1.0 ml,浓硫酸的体积为6.0 ml,反应时间为25 min;粗多糖含量在10～70μg/ml之间与吸光度有很好的线性关系,回归方程为Y=0.013 2X-0.085 7,相关系数R为0.999 6;试验方法的回收率为100.36%,RSD为1.26%,显色液在120min内稳定性良好。[结论]该方法准确可靠,为人参茎叶的进一步开发利用提供了依据。[ Objective ] To optimize the factor conditions of the polysaccimrides in ginseng stem leaves by phenol-sulfuric acid method. [ Method] Polysaccharides contents in ginseng stem leaves were detected by UV Speetrophotometry; detection condition was optimized by orthogonal test. [ Result ] The optimal wavelength was 485 nm for the determination of polysaceharides contents in ginseng stem leaves were detected by phenol-sulfuric acid method. The optimal color condition was A1 B2 CI , which were 1.0 ml of 6% phenol, 6.0 ml concentrated sulfuric acid, and 25 min reaction time. Crude polysaecharides had good linear relationship with absorbency within 10 - 70 μg/ml. The regression equation was Y = 0. 013 2X - 0. 085 7 ; correlation coefficient R was 0. 999 6 ; recovery rate of test method was 100.36% , and RSD was 1.26%. The coloured solution had good stability within 120 min. [ Conclusion ] This method was accurate and reliable, and provided references for the fur- ther development and utilization of ginseng stem leaves.

关 键 词：人参茎叶 苯酚-硫酸法 多糖 

分 类 号：S567.51[农业科学—中草药栽培]