作物病害高效生防菌株的定向快速筛选及其种类鉴定  

Fast Screening and Identification of High Efficiency Biocontrol Strains against Plant Disease

作　　者：石磊[1,2] 郭庆港[2] 李宝庆[2] 鹿秀云[2] 李社增[2] 王洪港 马平[2] 

机构地区：[1]河北农业大学植物保护学院,保河北定071000 [2]河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000 [3]河北省黄骅市城市管理局,河北黄骅061104

出　　处：《安徽农业科学》2012年第24期12068-12071,12096,共5页Journal of Anhui Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金项目(CARS-18-15);国家自然科学基金项目(30900962);"十二五"农村领域国家科技计划课题(2011AA10A205)

摘　　要：[目的]筛选和鉴定能产Bacillomycin D的芽孢杆菌。[方法]通过PCR技术对36株具有抑菌活性的芽孢杆菌进行检测,筛选出具有Bacillomycin D合成酶基因的菌株,并利用快速蛋白液相色谱技术(FPLC)对筛选出的菌株进行检测,最后通过16S rDNA和gyrB基因序列对这些菌株进行分子鉴定。[结果]共筛选出4株能产生Bacillomycin D的菌株,其中2株为萎缩芽孢杆菌(Bacillus atrophaeus),2株为解淀粉芽孢杆菌(B.amyloliquefaciens)。[结论]该研究为高效微生物农药的开发奠定了基础。[ Objective] The aim was to screen and identify Bacillomycin D producing strains of Bacillus. [ Method] PCR technique was used for detection the potential Bacillomycin D producing strains from 36 Bacillus wild type strains, and the isolated strains were detected by FPLC. Finally, the 165 rDNA and gyrB sequences were used to identify the Bacillomycin D producing strains. [ Result] Four Bacillomycin D produ- cing strains were selected, and strain CJT-2 and BNT-34 were identified as B. amyloliquefaciens, XJT-7 and CAB-1 were identified as B. atro- phaeus. [ Conclusion] The study lays a foundation for development of high efficiency microbial pesticides.

关 键 词：拮抗细菌 脂肽类抗生素 芽孢杆菌 筛选 鉴定 

分 类 号：S432.42[农业科学—植物病理学]