Characterization of a myrosinase cDNA from Brassica parachinensis and its defense role against Plutella xylostella after suppression  

作　　者：Guang Yang Ding-Feng wang Zhi-Quan Dong Qian-Liang Wang Gefu Wang-Pruski Min-Sheng You 

机构地区：[1]lnstitute of Applied Ecology,Fujian Agriculture and Forestry University,Fuzhou [2]Tea Research Institute,Fujian Academy of AgriculturalScience,Fu'an,Fujian [3]Institute of Prevention and Control of Professional Diseases,Fuzhou,China [4]Department of Plant and AnimalSciences,Nova Scotia Agricultural College,Truro,Nova Scotia,Canada

出　　处：《Insect Science》2012年第4期461-471,共11页昆虫科学（英文版）

摘　　要：In Brassicaceae, myrosinase catalyzes the hydrolysis of glucosinolate and plays an important role in anti-herbivore defense. We have cloned and characterized the full- length complementary DNA of myrosinase gene from Brassicaparachinensis that exhibits high sequence identity with myrosinase genes from other Brassica species. To investigate the role of this myrosinase in defense against the diamondback moth （Plutella xylostella）, we constructed an RNA-interference （RNAi） cassette expressing a double-stranded RNA that targeted myrosinase and transfected it into B. parachinensis. Myrosinase was sup- pressed in the resulting transgenic plants. Diamondback moth larvae feeding on transgenic plants had lower larval and pupal weights, longer pupal duration, and lower fecundity than those feeding on non-transgenic plants, suggesting that the diamondback moth has adapted to the glucosinolate-myrosinase defensive system. Therefore, the suppression of myrosinase is a potential approach for controlling the diamondback moth.In Brassicaceae, myrosinase catalyzes the hydrolysis of glucosinolate and plays an important role in anti-herbivore defense. We have cloned and characterized the full- length complementary DNA of myrosinase gene from Brassicaparachinensis that exhibits high sequence identity with myrosinase genes from other Brassica species. To investigate the role of this myrosinase in defense against the diamondback moth （Plutella xylostella）, we constructed an RNA-interference （RNAi） cassette expressing a double-stranded RNA that targeted myrosinase and transfected it into B. parachinensis. Myrosinase was sup- pressed in the resulting transgenic plants. Diamondback moth larvae feeding on transgenic plants had lower larval and pupal weights, longer pupal duration, and lower fecundity than those feeding on non-transgenic plants, suggesting that the diamondback moth has adapted to the glucosinolate-myrosinase defensive system. Therefore, the suppression of myrosinase is a potential approach for controlling the diamondback moth.

关 键 词：Brassica parachinensis MYROSINASE Plutella xylostella RNA interference 

分 类 号：S482.84[农业科学—农药学] TQ453.307[农业科学—植物保护]