吡哆胺和替米沙坦对血管紧张素Ⅱ诱导的大鼠心肌细胞氧化应激的影响  

Effect of Pyridoxamine and Telmisartan on Oxidative Stress of Rat Cardiac Myocytes Induced by Angiotensin Ⅱ

作　　者：陈建康[1] 朱鹏立[1] 杨津[1] 余惠珍[1] 林帆[1] 林虹[1] 郑炜平[1] 

机构地区：[1]福建省立医院干部特诊科,福州350001

出　　处：《中国医科大学学报》2012年第7期622-624,629,共4页Journal of China Medical University

基　　金：福建省自然科学基金资助项目(2010J01127);福建省卫生厅青年科研基金资助项目(2010213)

摘　　要：目的探讨吡哆胺和替米沙坦对血管紧张素Ⅱ诱导的大鼠心肌细胞氧化应激的影响。方法大鼠心肌细胞培养并随机分为正常对照组(C组),血管紧张素Ⅱ组(1×10-6mol/L,AngⅡ组),吡哆胺组[吡哆胺(10 mmol/L)+AngⅡ(1×10-6mol/L),P组],替米沙坦组[替米沙坦(1×10-6mol/L)+AngⅡ(1×10-6mol/L),T组],联合治疗组[替米沙坦(1×10-6mol/L)+吡哆胺(10 mmol/L)+AngⅡ(1×10-6mol/L),TP组]。流式细胞仪检测细胞上清液中的活性氧簇(ROS)浓度,硫代巴比妥酸法(TBA)测定丙二醛(MDA)浓度,黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活力,荧光实时定量PCR方法检测心肌细胞所表达的晚期糖基化终产物受体(RAGE)mRNA表达量。结果与AngⅡ组比较,T组、P组及TP组MDA、ROS浓度均显著降低、SOD活力显著升高(P<0.05);与T组比较,P组、TP组的ROS浓度明显降低、SOD活力明显升高(P<0.05);与AngⅡ组比较,T组、P组和TP组RAGE mRNA表达量明显降低(P<0.01);与T组、P组比较,TP组的RAGE mRNA表达进一步降低(P<0.05)。结论吡哆胺和替米沙坦协同下调大鼠心肌细胞RAGE mRNA的表达,二者均可改善氧化应激,但吡哆胺较替米沙坦强。Objective To investigate the protective effect of telmisartan and pyridoxamine on oxidative stress in cultured rat cardiac myocytes. Methods Cultured cardiac myocytes were divided into control group （C）, angiotensin Ⅱ （1× 10^-6 mol/L） group （Ang Ⅱ ）, pyri- doxamine group （pyridoxamine 10 mmol/L,P）,telmisartan group （telmisartan 1×10^-6 mol/L＋ Ang Ⅱ 1×10^-6 mol/L,T）,and T ＋ P ＋ an- giotensin H group （telmisaaan 1×10^-6 mol/L ＋ pyridoxamine 10 mmol/L＋Ang Ⅱ 1×10^-6 mol/L,TP）. Reactive oxygen species （ROS）genera- tion in cellular supematant was detected by flow cytometry. The maleic dialdehyde （MDA） was detected by thiobarbituric acid （TBA）. Xan- thine oxidase was performed to determine super oxide dismutase （SOD） in cellular supematant. Real-time quantitative PCR was used to mea- sure the mRNA expression level of receptor for advanced glycation end-products （RAGE）. Results Compared with the Ang Ⅱ group, the level of ROS and MDA was reduced,while SOD activity was increased in T,P and TP groups （P 〈 0.05）. Compared with the T group,the level of ROS was reduced,while SOD activity was increased in P and TP groups （P 〈 0.05）. Compared with the Ang H group,the mRNA expression level of RAGE was decreased significantly in T,P and TP groups （P 〈 0.01 ）. Compared with T and P groups,mRNA expression in RAGE was much lower in TP group （P 〈 0.05 ）. Conclusion There is a synergistic effect on reducing rat cardiac myocytes RAGE by combined use of telmisartan and pyfidoxamine. Pyridoxamine and telmisartan can improve oxidative stress in rat cardiac myocytes and pyri- doxamine is more effective than telmisartan.

关 键 词：吡哆胺 替米沙坦 氧化应激 晚期糖基化终产物受体 心肌细胞 

分 类 号：R544.1[医药卫生—心血管疾病]