地拉罗司对成年大鼠狭长窄蒂皮瓣DLL4、CD105表达及血管新生的影响  

Effect of deferasirox on DLL4 expression and angiogenesis in a slender narrow pedicle and random flap in

作　　者：冀宇[1] 赵天兰[1] 余道江[1] 徐子寒[1] 杨建新[1] 

机构地区：[1]苏州大学附属第二医院整形美容外科,215004

出　　处：《中华医学美学美容杂志》2012年第4期298-301,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：江苏省研究生创新培养项目（编号：CX09B-039Z）;江苏省苏州市青年科教兴卫项目（编号：SWKQ0915）

摘　　要：目的探讨地拉罗司对成年大鼠狭长窄蒂皮瓣Delta样配体4（DLL4）、CD105表达及血管新生的影响。方法健康成年SD大鼠20只随机分为地拉罗司组和对照组（n=10）。地拉罗司组每只大鼠采用地拉罗司100mg·kg^-1·d^-1连续灌胃1周，对照组采用等量生理盐水连续灌胃1周。灌胃停，止后，两组每只大鼠背部左右各设计1个蒂部为1cm×1cm、瓣部为3cm×3cm的狭长窄蒂乒乓球拍样随意型皮瓣模型。手术1周后分别取各个皮瓣蒂部、皮瓣部中间位置组织1cm×1cm，以10％甲醛溶液固定，免疫组织化学（SABC法）分别检测CD105和DLL4。结果地拉罗司组与对照绀相比,无论在蒂部或是在皮瓣部，大鼠皮瓣组织内CD105标记的新生血管均增多，DLL4表达的新，L血管均减少。两组中．皮瓣部组织中CD105、DLL4标记的新生血管均多于蒂部。结论地拉罗司促进狭长窄蒂皮瓣中的CD105表达、促进血管新生。并且可能与其抑制皮瓣Notch途径中关键蛋白DLL4的表达有关。Objective To investigate the effect of deferasirox on DLL4 expression and angiogen-esis in a narrow pedicle and random flap in rats. Methods Twenty SD rats were randomly divided into the deferagirox group and control group. Rats were subjected to deferagirox of 100 mg · kg^-1·d^-1 in the experimental groups, respectively and the same dose saline in the control group for 1 week. In each group, flap were created in the bilateral back of each rats. Ratio of length to width of tissue in the pedicle portion and the flap portion was 1 cm × 1 cm and 3 cm ×3 cm, respectively. The tissue samples were taken from the pedicle and the middle portions of the flap. The DI.L4 and CD105 expres-sion was also detected with immunohistochemistry （SABC）. Results Compared with comrol group, whatever in the pedicle portion or the middle portion, there was a significant increase of microvessels marked by CD105 and a significant decrease of flap microvessels stained by DIAA in the deferagirox group. In both groups, compared with the pedicle portion, there was a significant increase of mi crovessels marked by CD105 and DLL4 in the the middle portion. Conclusions Deferasirox can in-crease the CD105 expression and angiogenesis of the slender narrow pedicle random flap. This process might be related to the inhibition of DI.IA protein expression, which is significant in the notch signa ling pathway.

关 键 词：Delta样配体4 CD105 地拉罗司 随意型皮瓣 大鼠 

分 类 号：R965[医药卫生—药理学]