不同频率电针对晚孕大鼠内分泌激素的影响  

Effect of Electroacupuncture at Different Frequencies on Serum Estradiol and Progesterone Levels in Late-stage Pregnancy Rats

作　　者：朱玲[1,2] 杨芳[3] 金春兰[4] 马良宵[1] 陈滢如[4] 苑鸿雯[1] 朱江[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]煤炭总医院中医科,北京100028 [3]辽宁中医药大学,沈阳110032 [4]中国中医科学院针灸研究所,北京100700

出　　处：《针刺研究》2012年第4期281-285,共5页Acupuncture Research

基　　金：国家重点基础研究发展计划("973"计划)资助项目(No.2012CB 518506);教育部博士点基金资助项目(No.20090013110005)

摘　　要：目的:观察不同频率电针对晚孕大鼠血清雌二醇(E2)及孕酮(P)含量、血栓素B2(TXB2)及6-酮-前列腺素(6-keto-PGF1α)含量以及其比值的影响,探讨不同频率电针促晚孕大鼠分娩的机制。方法:健康成年晚孕Wistar大鼠66只,随机分正常组、模型组和4个不同频率电针组(2Hz、20Hz、50Hz、100Hz)。除正常组外均用托盘法造成大鼠妊娠模型。4个电针组于受孕第19日上午进行电针干预,取双侧"合谷""三阴交"穴,先电针"合谷"穴20min,再针"三阴交"5min,共留针25min。用放射免疫法测定血清E2、P、TXB2、6-keto-PGF1α含量,并计算E2/P、TXB2/6-keto-PGF1α比值。结果:与模型组比较,2Hz、20Hz电针组血清E2含量显著升高(P<0.05)。各电针组血清P含量比较,100Hz较20Hz和50Hz组显著增高(P<0.05)。与模型组比较,2 Hz、50 Hz电针组血清TXB2含量显著升高(P<0.01,P<0.05)。结论:2Hz、50Hz频率电针可升高血清TXB2含量及E2/P比值,提示2Hz和50Hz电针更有利于触发宫缩,加快产程。Objective To observe the effect of etectroacupuncture （EA） at different frequencies on serum estradiot （E2）, progesterone （P）, thromboxance B2 （TXB2）, 6-Keton-prostagiandin F 1 alpha （6-keto-PGFl=）contents in late pregnancy rats so as to study its mechanisms underlying labor promotion. Methods A total of 66 late-stage pregnant Wistar rats were randomly divided into 6 groups： normal control, late-pregnancy model （model）, 2 Hz-EA, 20 Hz-EA, 50 Hz-EA and 100 Hz-EA groups, with 11 rats in each group. On the 19TM day after pregnancy, EA（2 Hz, 20 Hz, 50 Hz, 100 Hz, 0. 1 mA） was applied to bilateral ＂Hegu＂（LI 4） for 20 min first and to bilateral ＂Sanyinjiao＂（SP 6） for 5 min afterwards. Serum E2, P, TXB2 and 6-keto- PGF1α contents were assayed by radioimmunoassay, and the ratios of E2/P and TXB2/6-keto-PGFlαwere calculated. Results Compared to the normal control group, serum E2 contents in 2 Hz-EA, 20 Hz-EA, 50 Hz-EA and 100 Hz-EA groups were increased significantly（ P〈0.001, P〈0. 01, P〈0. 05）. Compared to the model group, serum E2 levels in the 2 Hz-EA and 20 Hz-EA groups were up-regulated further significantly（P〈0.05）. Comparison among the four EA groups showed that serum P level in the 100 Hz- EA group was significantly higher than those in the 20 Hz-EA and 50 Hz-EA groups（P〈0.05）. Compared to the normal control group, the contents of serum TXBz and the ratios of E2/P in both 2 Hz-EA and 50 Hz-EA groups were significantly higher（ P〈0. 05, P〈0. 001, P〈0. 01 ）. Conclusion Both 2 Hz and 20 Hz EA can effectively increase serum E2. content, and 2 Hz and 50 Hz EA can raise serum TXB2 and E2/P levels in late pregnancy rats, which may contribute to their effects in strengthening the uterine contractility to promote labor by regulating hormone secretion.

关 键 词：晚孕 电针 刺激频率 内分泌激素 合谷 三阴交 

分 类 号：R246.3[医药卫生—针灸推拿学]