银杏叶提取物抗动脉粥样硬化的实验研究  

Study of the anti-atherosclerosis effects of extract of Ginkgo Biloba

作　　者：万冬宇[1] 黄维义[2] 刘全未[3] 

机构地区：[1]郑州人民医院,郑州450003 [2]泸州医学院附属医院 [3]四川内江市第二人民医院

出　　处：《现代预防医学》2012年第15期3908-3910,共3页Modern Preventive Medicine

基　　金：四川省自然科学研究基金项目(04JY029-021-3)

摘　　要：目的探讨银杏叶提取物(effects of Extract of Ginkgo Biloba EGB)是否通过诱导血红素氧合酶-1(HO-1)表达发挥抗动脉样硬化(atherosclerosis AS)作用。方法选用雄性Wistar大鼠40只,随机均分为A、B、C、D、E5组,A组给普通饲料喂养,其余各组均给高脂饲料喂养,C组、D组、E组分别给予腹腔注射EGB 30 mg/kg、锌原卟啉(ZnPPⅨ)7.5 mg/kg、EGB 30 mg/kg+ZnPPⅨ7.5 mg/kg,每两天1次,A组及B组则同法注射等量生理盐水。12周后检测主动脉HO-1蛋白表达量、HO-1蛋白活力及AS病变程度。结果与A组相比,B组及C组HO-1蛋白表达量及活力均明显升高,C组升高尤为显著,D组及E组HO-1蛋白表达量及活力则显著降低。与B组比较,C组AS程度明显减轻,而D组及E组AS程度则显著加重。结论 EGB主要通过诱导HO-1表达发挥显著的抗AS作用。OBJECTIVE To explore whether the extract of Ginkgo Biloba produce （EGB） induces heme oxygenase-1 （HO-1） expression to play an anti-atherosclerosis role. METHODS 40 male Wista rats were randomly divided into group A, group B, group C, group D and group E. Group A was given normal diet while the other groups received high-fat diet. Group C, group D, group E were intra-peritoneal injected with EGB （30mg/kg） , ZnPP IX （7.5mg/kg） , EGB （30mg/kg） ＋ ZnPP IX （7.5mg/kg） respectively. Group A, group B were intra-peritoneal injected with equal volume saline. 12 weeks later, the ex- pression and activity of aorta HO-1 protein and the atherosclerosis degree were examined. RESULTS Compared with group A, the expression and activity of HO-1 protein were increased significantly （P 〈 0.05 respectively） in group B and group C but de- creased significantly （P 〈 0.05 respectively） in group D and group E （P 〈 0.05 respectively）. Compared with group B, the atherosclerosis degree in group C were reduced obviously （P 〈 0.05） but sharpened significantly in group D and group E （P 〈 0.05 respectively）. CONCLUSION The marked anti-atherosclerosis role of EGB is mainly mediated by inducing heme oxyge- nase-1 expression.

关 键 词：银杏叶提取物 血红素氧合酶-1 动脉粥样硬化 大鼠 

分 类 号：R969.4[医药卫生—药理学]