尿生殖窦植入致大鼠良性前列腺增生的组织形态学研究  

Benign prostate hyperplasia induced by urogenital sinus implantation in rats:A histomorphological study

作　　者：申青[1] 朱着[1] 朱柳[1] 袁牧[1] 

机构地区：[1]广州医学院药物研究中心,广东广州510182

出　　处：《中华男科学杂志》2012年第8期703-709,共7页National Journal of Andrology

基　　金：广东省高等学校科技创新重点项目(cxzd1127)~~

摘　　要：目的:采用尿生殖窦植入法建立大鼠良性前列腺增生模型并进行组织形态学半定量及免疫组化研究。方法:7周龄雄性SD大鼠32只,随机分为4组:假手术对照组,尿生殖窦模型2周组,尿生殖窦模型3周组,尿生殖窦模型6周组。尿生殖窦植入各模型组大鼠,分别饲养指定周期后,分离各组大鼠前列腺组织,测量湿重及体积,右侧前列腺腹前叶切片并进行半定量组织形态学测量,采用免疫组化法检测上皮、间质、平滑肌中相关表达因子。结果:各模型组大鼠右侧前列腺腹叶湿重及指数、体积及指数随模型培养时间延长而增加(P<0.05),间质面积比例、间质相对总体积随时间延长显著增加(P<0.01),3周模型组的间质平均比例高达75.32%;模型组上皮相对总体积随时间延长亦增加。与假手术对照组相比,腺腔面积、上皮面积比例,腺腔面积比例减少。广谱细胞角蛋白免疫组化染色阳性颗粒定位于上皮细胞,波形蛋白于间质细胞丰富表达,平滑肌肌动蛋白于腺腔周围表达。结论:大鼠尿生殖窦良性前列腺增生模型以间质增生为主,相关因子表达部位与人类良性前列腺增生相似。Objective： To study the histomorphological and immunochemical characteristics of benign prostate hyperplasia （BPH） induced by urogenital sinus （UGS） implantation in the rat model. Methods ： We randomized 32 seven-week old male SD rats into a sham operation control and three （2-, 3- and 6-week） UGS model groups. We made the UGS model by implanting two urogenital sinuses from homogeneous male rat embryos into the host rats fight anterior lobe, and killed the model rats 2, 3 and 6 weeks later for measuring the wet weight, volume and mieromorphological parameters of the right anterior lobe and detecting the factors expressed in the epithelium, stroma and smooth muscle by immunochemistry. Results： The model rats showed significant increases in the wet weight, volume and relevant indexes of the right anterior lobe （ P 〈 0.05 ）, as well as in the proportion of stromal area and relative stroreal volume （ P 〈 0.01 ） with the prolonging of time. The mean stromal proportion of the 3-week models was as high as 75.32%. There was also a time-dependent increase in the relative total volume of epithelia in the model groups. The luminal area and the proportions of the luminal and epithelial volumes were obviously reduced in the model rats as compared with the sham operation controls. Pan-cytokeratin positive particles were located in epithelial cells, vimentins abundantly expressed in mesenchymocytes, and a-smooth muscle actins expressed around the lumen of the gland. Conclusion ： BPH induced by urogenital sinus implantation in rats is typically stromal hyperplasia, and the locations of related factors are similar to those in men with BPH.

关 键 词：前列腺间质增生 动物模型 形态计量学 尿生殖窦 

分 类 号：R697.32[医药卫生—泌尿科学] R332[医药卫生—外科学]