E-钙粘蛋白抗体对小鼠早期胚胎发育的影响  

Effects of E-cadherin Antibody on Mouse Early Embryo Development

作　　者：吴兴龙[1] 王棉娟[2] 胡春超[1] 王鹏博[1] 李相运[1] 

机构地区：[1]河北农业大学动物科技学院,保定071000 [2]河北农业大学校医院,保定071000

出　　处：《畜牧兽医学报》2012年第8期1222-1229,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30871790);河北省自然科学基金(C2009000600)

摘　　要：旨在研究E-钙粘蛋白抗体(ECCD-1)对小鼠早期胚胎体内外发育的影响。在体外培养小鼠8-细胞胚胎的培养液中,分别添加不同浓度的ECCD-1,通过胚胎形态观察、碱性磷酸酶染色、多能性因子免疫染色、体外贴壁培养、嵌合体制备以及体内移植试验检测ECCD-1对小鼠胚胎发育能力的影响。结果表明,ECCD-1抗体延迟胚胎致密化和囊胚腔形成,但内细胞团并未受到影响,胚胎碱性磷酸酶和SSEA-1、Oct-4、Sox2、Nanog 4种多能性因子的免疫染色都呈阳性,此外,ECCD-1抗体也不影响胚胎的体外贴壁生长、体内移植着床以及嵌合体的制备。这些数据表明,ECCD-1抗体延迟胚胎致密化但并不影响胚胎的体内外发育能力。To investigate the effects of E-cadherin antibody（ECCD-1） on mouse embryo development,ECCD-1 antibody with different concentrations was added into KSOM media when mouse 8-cell embryos were cultured in vitro,and then the developmental potential of ECCD-1-treated embryos were detected by morphology,alkaline phosphatase activity,pluripotent marker（SSEA-1,Oct-4,Sox-2,Nanog） immunostaining,attachment culture in vitro,implantation in vivo,and chimera production.The result showed that ECCD-1 significantly delayed embryo compaction and blastocoele formation.ECCD-1-treated embryos had obvious inner cell mass,positive alkaline phosphatase and pluripotent markers,normal attachment growth in vitro and implantation in vivo.In addition,the chimera production was also improved when 8-cell embryos injected by embryonic stem cells were treated with ECCD-1.The results indicated that compaction delay by ECCD-1 antibody did not affect embryo development potential.

关 键 词：小鼠 E-钙粘蛋白抗体 胚胎致密化 发育能力 

分 类 号：Q132.4[生物学—普通生物学]