脂质体包被鸡IL-18可增强新城疫活疫苗的免疫效果  

Effect of Mature Chicken Interleukin-18 Protein on Its Immunoenhancement on Newcastle Disease Vaccine

作　　者：张海滨[1,2] 周明东[2] 周霞[2] 胡敬东[2] 赵宏坤[1,2] 

机构地区：[1]青岛农业大学食品科学与工程学院山东省肉类食品质量安全工程技术研究中心,青岛266109 [2]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2012年第8期1287-1291,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"十二五"科技支撑项目(2012BAK17B05);国家肉鸡产业技术体系建设项目;山东省科技支撑计划项目(2007GG20009001)

摘　　要：首次将重组鸡白细胞介素18(mChIL-18)用脂质体包被,将不同浓度的mChIL-18脂质体联合新城疫疫苗对鸡群进行免疫,观察其对新城疫疫苗的免疫增强作用。1日龄SPF鸡分为6组,分别为空白对照组、单纯疫苗组、新城疫疫苗+0.2mL mChIL-18组、新城疫疫苗+0.1mL mChIL-18组、新城疫疫苗+0.3mL mChIL-18组和新城疫疫苗+空载体对照组。所有免疫组鸡于7日龄免疫,免疫后每周抽取外周血及外周抗凝血,应用血凝抑制试验和流式细胞仪来检测免疫鸡的HI抗体水平及CD4+和CD8+T淋巴细胞的变化情况。并于最后一次采血后,对各组试验鸡应用F48E9标准强毒进行攻击(100ELD50.只-1)。结果表明mChIL-18脂质体的最佳剂量为0.2mL.只-1,其不仅能够显著增强机体的细胞免疫和体液免疫,而且还可以明显提高新城疫疫苗的保护率。The liposome-encapsulated mChIL-18 was firstly applied on its immunoenhancement for Newcastle disease vaccine with different concentrations.In the experiment,one-day-old SPF chickens were randomly divided into six groups,the blank control group,the NDV vaccine group,the NDV vaccine plus 0.2 mL mChIL-18 group,the NDV vaccine plus 0.1 mL mChIL-18 group,the NDV vaccine plus 0.3 mL mChIL-18 group and the NDV vaccine plus vector control group.All groups were immunized on the seven days old.After that,peripheral blood was collected with anticoagulation,and the antibody level,CD4＋ and CD8＋ T lympholeukocytes were detected by HI and FACS at each week,respectively.Standard Virulent Strain F48E9（100 times ELD50 per chicken） was given to the chickens at last vaccination.The results indicated that the best concentration of mChIL-18 liposomes is 0.2 mL per chicken.The mChIL-18 liposomes can not only enhance the cellular immune response significantly,but also enhance the humoral response,improving the protective rate of ND vaccine.

关 键 词：mChIL-18 脂质体 新城疫疫苗 免疫增强 

分 类 号：S852.4[农业科学—基础兽医学]