丹参注射液诱导类风湿关节炎成纤维样滑膜细胞NF-κB表达研究  

The changes of NF-κB promote rheumatoid arthritis fibroblast-like synoviocytes apoptosis by Danshen injection

作　　者：刘青松[1,2,3] 朱兴春[2] 蒋红[1,3] 蔡燕[1,2,3] 袁国华[1,3] 杨明辉[1,2,3] 张均[2] 李君安[2] 唐中[1,2,3] 

机构地区：[1]川北医学院附属医院风湿免疫研究所 [2]川北医学院附属医院检验科 [3]川北医学院附属医院风湿与感染免疫实验室

出　　处：《中国临床药理学与治疗学》2012年第8期846-850,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：四川省卫生厅科研项目(090141;100151);川北医学院重点实验室开放基金[KFJJ(09)-04]

摘　　要：目的:探讨丹参注射液对类风湿关节炎(RA)患者成纤维样滑膜细胞(FLSs)P65和P50表达及其在细胞内定位的影响。方法:RA患者关节液原代培养获取FLSs,终浓度为0、0.2和0.4mg/mL的丹参注射液作用24h后,采用细胞免疫化学方法对P65和P50表达定位进行分析;提取核蛋白,采用免疫印迹方法检测P65和P50表达。结果:细胞免疫化学结果显示,在无药物刺激的条件下,P65和P50均主要集中在细胞质中;当经0.2mg/mL丹参注射液作用24h,P65和P50均有向细胞核转移趋势;当丹参注射液浓度为0.4mg/mL时,P65和P50在细胞核中有明显聚集。免疫印迹结果表明,P65和P50在细胞核中表达量随丹参注射液浓度升高而增加,并当丹参注射液浓度为0.4mg/mL时,二者的表达相比药物作用前显著增加,具有统计学意义(P<0.05)。结论:丹参注射液可调节RAFLSs P65和P50从细胞质向细胞核转移。AIM. To investigate the changes of expressions and the location of P65 and P50 in rheumatoid arthritis fibrobtast-like synoviocytes （FLSs） by Danshen injection. METHODS： Fibroblast-like synovial cells obtained from synovial fluid by primary culture, and then treated with final concentrations of 0, 0.2 and 0.4 mg/mL of Danshen injection for 24 h. The expressions and location of P65 and PS0 were detected with immunocytochemistry and Western blot. RESULTS.. Immunocytochemistry showed that in the absence of drugs under the conditions of stimulation, either P65 or P50 was mainly concentrated in the cytoplasm, when the final concentration of Danshen injection reaches 0.2 mg/mL, both of them had a tendency to nuclear transfer, when the final concentration of Dansh-en injection to 0.4 mg/mL, both of them significantly accumulated in the nucleus. Western blot results showed that the expressions of P65 and P50 in the nucleus increased with the elevated concentration of Danshen injection, and when the concentration of Danshen injection reach to 0.4 mg/mL, the expressions of both of them increased significantly （P 〈 0.05, n = 4）. CONCLUSION： Danshen injection can adjust rheumatoid arthritis fibroblast-like synoviocytes P65 and P50 from the cytoplasm to the cell nuclear transfer, and thus promote apoptosis of fibroblast-like synoviocytes.

关 键 词：丹参 类风湿关节炎 成纤维样滑膜细 胞 NF—κB 

分 类 号：R593.22[医药卫生—内科学]