中国肾移植患者钙蛋白酶10基因多态性与移植后糖尿病的相关性研究  

Calpain10 gene polymorphisms are associated with posttransplantation diabetes mellitus in Chinese renal allograft recipients

作　　者：余爱荣[1] 范星[2] 刘慧明[1] 辛华雯[1] 

机构地区：[1]广州军区武汉总医院临床药理科 [2]广州军区武汉总医院肾病内科

出　　处：《中国临床药理学与治疗学》2012年第8期901-906,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湖北省自然科学基金计划资助项目(2009CDB010)

摘　　要：目的:探讨中国肾移植患者钙蛋白酶10(CAPN10)基因多态性与移植后糖尿病(PTDM)的相关性。方法:采用等位基因特异性PCR、限制性片断长度多态性PCR(PCR-RFLP)分别检测了97例PTDM患者(PTDM组)和301例未发生PTDM的肾移植患者(对照组)的CAPN10基因SNP-19、SNP-43、SNP-63的基因型,采用lo-gistic回归分析该基因多态性与PTDM的相关性。结果:PTDM组患者SNP-19的11+12基因型频率和SNP-43的GG基因型频率明显高于对照组(P<0.05)。用性别、移植时年龄、体重和BMI进行校正后,SNP-19的11基因型和12基因型携带者移植术后发生PTDM的风险分别是22基因型的1.502倍(OR=1.502,95%CI:1.016～2.347,P=0.048)和1.764倍(OR=1.764,95%CI:1.055～2.947,P=0.030),SNP-43的GG基因型携带者移植术后发生PTDM的风险是AA和GA基因型患者的2.19倍(OR=2.190,95%CI:1.047～3.473,P=0.044),SNP-63与PTDM的发生无明显相关性(P>0.05)。结论:CAPN10基因SNP-19的1等位基因和SNP-43的GG基因型是肾移植后发生PTDM的独立危险因素。AIM： To investigate the association between the Calpainl0 gene polymorphism and the risk of PTDM in Chinese renal allograft recipients. METHODS. Three single nucleotide polymorphisms （CAPN10 gene SNP-19, SNP- 43, SNP-63） were genotyped in the cohort, which consisted of 97 renal allograft recipients with PTDM （PTDM group） and 301 renal allograft recipients without PTDM （ control group）. The genotypes of polymorphisms were performed by allele specific polymerase chain reaction （ ASPCR ）, PCR-restriction fragment length polymorphism （PCR-RFLP）. Logistic regression test was used to identify risk factors for PTDM development and calculate the odds ratio. RESULTS.The 11 and 12 genotypes of SNP-19 and the GG genotypes of SNP-43 were more common in patients with PTDM than those without PTDM （P 〈 0.05）. After adjustmentsfor age, sex, body weight and BMI, the effect of genotype remained significant （11 vs 22, OR= 1.502, 95%CI： 1.016-2.347, P=0.048; 12 vs 22, OR=1. 764, 95%CI： 1. 055-2. 947, P= 0. 030） in SNP-19 and the patients carrying genotype GG had higher risk comparison with carriers with genotype AA or GA（OR=2. 190, 95% CI： 1.047-3.473, P=0.044）in SNP-43. But SNP-63 was not association with PTDM. CONCLUSION： The 1-allele in SNP-19 and GG genotype in SNP-43 of CAPN10 gene are the independent risk factors of PTDM in Chinese renal allograft recipients.

关 键 词：肾移植患者 肾移植术后糖尿病 危险因 素 钙蛋白酶10基因 单核苷酸多态性 

分 类 号：R968[医药卫生—药理学]