丹参酮ⅡA预处理对大鼠心肌缺血再灌注损伤的作用研究  

Effect of Tanshinone ⅡA pretreatment on myocardial ischemia /reperfusion injury in rats

作　　者：吴晓倩[1] 何丽姗[1] 

机构地区：[1]广州医学院药理教研室,广东广州510182

出　　处：《热带医学杂志》2012年第8期917-919,F0002,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(81001436);广东省自然科学基金(10451018201005279);广东高校优秀青年创新人才培养项目(LYM10110);广州市首届"羊城学者"科研项目(10A018G);广州市属高校科研项目(10A157)

摘　　要：目的探讨丹参酮ⅡA对大鼠心肌缺血再灌注损伤的作用。方法雄性SD大鼠随机分为假手术组、模型组、丹参酮ⅡA预处理组,后两组进行冠状动脉左前降支结扎30min后再灌注120min。期间全程监测心率和血流动力学指标。实验结束后取心脏做TTC染色及HE染色,观察心肌梗死面积和组织学改变。结果模型组大鼠心脏功能明显下降并发生心肌梗死提示造模成功。与模型组比较,丹参酮ⅡA组在缺血30min,再灌注30、60、120min各时间点,左心室收缩末压、左室内压最大上升及下降速率明显增高、心率基本恢复正常。组织学染色显示,与模型组比较,丹参酮ⅡA组心肌梗死区面积降低、心肌细胞变性坏死程度明显减轻。结论丹参酮ⅡA预处理对大鼠心肌缺血再灌注损伤具有保护作用。Objective To investigate the effect of Tanshinone ⅡA on myocardial ischemia/reperfusion injury in rats. Methods Male SD rats were randomized to Sham operated group, ischemia/reperfusion （I/R） group and Tanshinone ⅡA group. Rats in I/R group and Tanshinone ⅡA group were subjected to legation of left anterior descending coronary artery for 30 minutes and 120 min of reperfusion. The hemodynamics were monitored during the whole process. TTC and HE staining were performed to examine the infarct size and the histology of hearts. Result Compared with I/R group, the cardiac hemodynamics were significantly improved after TanshinoneⅡA pretreatment, and Tanshinone Ⅱ A reduced the infarct size significantly compared with I/R group. Conclusion TanshinoneⅡ A pretreatment exerted protection against myocardial ischemia/reperfusion injury.

关 键 词：缺血再灌注 丹参酮ⅡA 心肌缺血 

分 类 号：R542.2[医药卫生—心血管疾病]