双波长梯度洗脱高效液相色谱法测定冲和软膏中芍药苷和蛇床子素的含量  

Simultaneous determination of peoniflorin and osthole in Chonghe ointment by HPLC with gradient elution and dual wavelength detection

作　　者：孙士真[1] 王亚静[1] 张德芹[1] 田慧[1] 彭淑娟[1] 王艳明[1] 刘芳[1] 

机构地区：[1]天津中医药大学,现代中药发现与制剂技术教育部工程研究中心,天津300193

出　　处：《中国新药杂志》2012年第14期1676-1680,共5页Chinese Journal of New Drugs

基　　金：天津市高校双五科技计划项目(SW20080007)

摘　　要：目的:建立RP-HPLC法同时测定冲和软膏中芍药苷和蛇床子素含量的方法。方法:色谱柱为Phenomisl C18柱(150 mm×4.6 mm5,μm);以乙腈-水为流动相进行梯度洗脱;流速为1.0 mL·min-1;柱温为35℃;检测波长为230和330 nm。结果:芍药苷与蛇床子素的线性范围分别为3.12～249.8μg·mL-1(r=0.999 8)和1.54～123μg·mL-1(r=0.999 9);平均加样回收率分别为101.0%(RSD=1.07%,n=9)和100.8%(RSD=1.21%,n=9)。结论:本方法操作简便,测定结果准确,重复性好,可作为该产品质量控制的方法。Objective：To establish a HPLC method for the determination of peoniflorin and osthole in Chonghe Ointment. Methods：The HPLC analysis was performed on a Phenomisl C18 column （150 mm× 4.6 mm, 5 μm） with acetonitrile-water as mobile phase in gradient elution; the flow rate was 1.0 mL· min^-1 The column temperature was 35 ℃. The detection wavelengths were set at 230 and 330 nm. Results：The linear ranges of peaoniflorin and osthole were 3.12 - 249.8 μg· mL ^- 1 （ r = 0. 999 8 ） and 1.54 - 123 μg·mL ^-1 （ r = 0. 999 9）. The aver- age recoveries were 101.0% （ RSD = 1.07% , n = 9 ） and 100.8% （ RSD = 1.21% , n = 9 ）, respectively. Conclusion：The method is convenient, rapid and accurate. It can be used in the quality control of Chonghe Ointment.

关 键 词：RP-HPLC法 梯度洗脱 双波长检测 冲和软膏 芍药苷 蛇床子素 

分 类 号：R927.2[医药卫生—药学]