HPCE法手性拆分(R)-和(S)-3-羟基戊二酸乙酯  

Chiral resolution of(R)-and(S)-ethyl 3-hydroxyglutarate by HPCE

作　　者：章申申[1] 董华平[1] 胡六江[1] 徐新[1] 

机构地区：[1]绍兴文理学院化学化工学院,浙江绍兴312000

出　　处：《化学试剂》2012年第8期743-745,共3页Chemical Reagents

基　　金：浙江省科技厅科技计划分析测试基金资助项目(2011C37012);绍兴市科技计划项目重点科研资助项目(2011A21056);浙江省实验教学示范中心实验教学改革项目资助项目;绍兴市教育科学规划课题资助项目(SGJ12008)

摘　　要：以β-环糊精(β-CD)为手性选择剂,采用毛细管区带电泳(CZE)对(R)-和(S)-3-羟基戊二酸乙酯进行手性拆分。通过考察手性选择剂浓度、电泳缓冲液pH、柱温以及分离电压对分离的影响,建立了比较理想的毛细管电泳手性拆分方法。在30 mmol/Lβ-CD、pH 5.0、柱温25℃以及分离电压20 kV分离条件下,(R)-和(S)-3-羟基戊二酸乙酯得到了完全分离。该分离方法具有较好的重复性和稳定性。(R)-And(S)-ethyl 3-hydroxyglutarate were successfully separated into two enantiomers by high performance capillary electrophoresis after β-cyclodextrin(β-CD) was introduced as a chiral selector.The ideally chiral resolution condition was established by investigation of the effects of the concentration of β-CD,pH of buffer,column temperature and separation voltage on the separation efficiency.The optimum conditions were 30 mmol/L of β-CD,pH 5.0,the voltage of 20 kV at 25 ℃.The results showed that this separation method was reproducible,reliable and stable.

关 键 词：毛细管电泳 手性拆分 (R)-3-羟基戊二酸乙酯 -β-环糊精 

分 类 号：O657.8[理学—分析化学]