红芪替代复芪止汗颗粒中的黄芪对免疫抑制小鼠细胞免疫作用的差异研究  

Differences of Hedysari Radix and Astragali Radix alternative in Fuqi Zhihan Granules on immunity in immunosuppressed mice

作　　者：张李峰[1] 卫东锋[1] 赵春燕[2] 程卫东[1,3] 桂曼曼[1] 李雪嫣[1] 

机构地区：[1]兰州大学基础医学院,甘肃兰州730000 [2]兰州大学药学院,甘肃兰州730000 [3]北京师范大学资源学院,北京100009

出　　处：《中成药》2012年第8期1415-1420,共6页Chinese Traditional Patent Medicine

基　　金：中国博士后科学基金资助(20090450017);中国博士后科学基金特别资助(201003073);教育部高等学校博士点基金项目(200807330006)

摘　　要：目的比较复芪止汗颗粒中用红芪与用黄芪对免疫抑制小鼠的细胞免疫调节作用。方法小鼠腹腔注射环磷酰胺建立免疫抑制小鼠模型,以相同剂量的红芪替换复芪止汗颗粒中的黄芪后,分别灌胃,通过测定各组的巨噬细胞体外吞噬中性红能力、脾淋巴细胞杀伤活性、T淋巴细胞亚群百分比、血清IL-1β的质量浓度比较两者对环磷酰胺的拮抗作用。结果环磷酰胺免疫抑制小鼠经复芪止汗颗粒和红芪替换黄芪后的复芪止汗颗粒作用后血清中IL-1β质量分数明显增加;含红芪和黄芪的复芪止汗颗粒通过不同程度地增加小鼠淋巴细胞增殖能力、杀伤活性、T淋巴细胞亚群百分比和腹腔巨噬细胞的吞噬功能发挥拮抗作用。含红芪的复芪止汗颗粒在提高环磷酰胺免疫抑制小鼠的T淋巴细胞增殖能力和血清IL-1β质量分数方面优于含黄芪的复芪止汗颗粒。结论红芪与黄芪调节细胞免疫方面具有相似作用,且在恢复T淋巴细胞增殖能力方面略优于黄芪。AIM To comparatively study the differences of Fuqi Zhihan Granules on immunosuppressed mice in cellular immunity in the substitution of Hedysari Radix for Astragali Radix. METHODS Mice injected intraperitoneally with cyclophosphamide were modeled into the immunosuppressed mouse and intragastrically administered with the same dose of Hedysari Radix replaced by Astragali Radix in Fuqi Zhihan Granules through the determination of macrophages in vitro macrophage phagocytic capacity, spleen lymphocyte killing activity, the percentage of T lymphocyte subsets, serum IL-1 β level to compare both cyclophosphamide antagonism. RESULTS Fuqi Zhihan Granules containing Hedysari Radix and Astragali Radix could significantly increase the level of IL-1β, and antagonize immunosuppressive effect caused by cyclophosphamide, concluding promoting T lymphocyte proliferation, cytotoxic activity of NK cell, quantity of T lymphocyte subgroup, and phagocytosis of the macrophage of varying degrees. Hedysari Radix was superb to Astragali Radix in Fuqi Zhihan Granules in raising T lymphocyte proliferation and IL-1β level. CONCLUSION Hedysari Radix has a similar role in cell immunity regulation with Astragali Radix, and Hedysari Radix has better effect in T cell proliferation.

关 键 词：红芪 黄芪 复芪止汗颗粒 免疫抑制 细胞免疫 

分 类 号：R285.5[医药卫生—中药学]