氯胺酮对脂多糖诱导人脐静脉内皮细胞核因子-κB表达的影响  

Effects of Ketamine on lipopolysaccharide-induced nuclear factor-kappaB expression of human umbilical vein endothelial cells

作　　者：高峰[1] 罗玉琳[1] 郁葱[1] 吴雨佳[1] 

机构地区：[1]重庆医科大学附属口腔医院麻醉科,重庆401147

出　　处：《重庆医科大学学报》2012年第7期599-601,共3页Journal of Chongqing Medical University

基　　金：重庆市教委科学技术研究资助项目(编号:kj080308)

摘　　要：目的:初步探讨胺酮对脂多糖(Lipopolysaccharides,LPS)作用下人脐静脉内皮细胞(Human umbilical vein endothelialcells,HUVECs)核因子-κB(Nuclear factor-kappa B,NF-κB)易位活化的影响及氯胺酮的抗炎效应。方法:HUVECs分为4组:C组(空白对照组)、L组(加入1μg/ml LPS)、KL1组(3μg/ml的氯胺酮+1μg/ml LPS)、KL2组(30μg/ml的氯胺酮+1μg/ml LPS)。采用免疫印记法(Western blot)、反转录聚合酶链式反应(Reverse transcription polymerase chain reaction,RT-PCR)和HPIAS2000图象分析系统,测定NF-κB p65亚基的易位活化表达。结果:KL1组、KL2组能抑制LPS作用下HUVECs内的NF-κB p65易位表达(P<0.05)。结论:LPS可引起人脐静脉内皮细胞核因子-κB的表达,而氯胺酮可以抑制其作用,且其抑制作用可能与剂量呈正相关。氯胺酮的抗炎机制可能是在核因子-κB转录水平上实现的。Objective：To investigate the effects of Ketamine on lipopolysaccharide（LPS）-induced translocation of nuclear factor-kappaB（NF-κB） in human umbilical vein endothelial cell nuclei and to explore it’s anti-inflammatory action.Methods：Human umbilical vein endothelial cells（HUVECs） were divided into four groups：group C（blank control group）,group L（1 μg/ml LPS was added）,group KL1（3 μg/ml ketamine and 1 μg/ml LPS were added） and group KL2（30 μg/ml ketamine and 1 μg/ml LPS were added）.The tanslocation and expressions of NF-κB p65 in endothelial cell nuclei were detected by Western blot,reverse transcription polymerase chain reaction（RT-PCR） and image analysis system of HPIAS 2000.Results：The tanslocation and expression of NF-κB p65 in endothelial cell nuclei induced by LPS were reduced in group KL1 and KL2.Conclusions：The tanslocation and expression of NF-κB p65 in endothelial cell nuclei can be induced by LPS and Ketamine can suppress the activation.There are positive correlations between Ketamine concentration and its inhibitory effect.The anti-inflammatory effect of Ketamine may be achieved by the translocation of NF-κB.

关 键 词：氯胺酮 脂多糖 核因子-ΚB 脐静脉 

分 类 号：R614.2[医药卫生—麻醉学]