石墨烯-Nafion-纳米金增敏印刷电极伏安法测定青藤碱  

Determination of sinomenine by graphene sheetNafion-gold nanopartical-sensitized screen-printed carbon electrodes for voltammeter

作　　者：杨欣[1,2] 陈迪钊[1,2] 林红卫[1,2] 袁吉[1] 李龙飞[1] 

机构地区：[1]怀化学院化学与化学工程系,怀化418000 [2]民族药用植物资源研究与利用湖南省重点实验室,怀化418000

出　　处：《药物分析杂志》2012年第8期1435-1439,1430,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：怀化学院大学生研究性学习和创新性实验项目(2011-16);湖南省高校"中药制剂工程与质量控制"产学研合作示范基地建设项目;怀化学院校级科研课题(HHUY2011-08);湖南省自然科学基金(11JJ6011)

摘　　要：目的:采用石墨烯(GS)-Nafion-纳米金(Au)增敏印刷电极(SPCEs)建立青藤碱(SN)的测定方法,探讨SN在修饰电极上的电化学行为。方法:将GS-Nafion溶液涂覆于SPCEs表面制得GS-Nafion︱SPCEs电极,然后于上述电极表面化学镀Au制得Au/GS-Nafion︱SPCEs电极,在B-R缓冲液中,采用循环伏安(CV)和示差脉冲伏安(DPV)法研究SN的电化学性质。结果:在优化的实验条件下,DPV氧化峰电流与SN浓度在5.0×10-7～1.0×10-4mol.L-1范围内呈良好的线性关系,检出限为2.2×10-7mol.L-1。结论:该修饰电极价格低廉,制备容易,样品用量少,抗干扰性强,可用于正清风痛宁注射液中SN含量测定。Objective：To investigate the electrochemical behavior of sinomenine（SN） by screen-printed carbon electrodes（SPCEs） sensitized by graphene sheets（GS）,Nafion and gold nanoparticals（Au）.Methods：SPCEs was coated by GS-Nafion solution and Au was painted on its surface to fabricate Au/GS-Nafion︱SPCEs electrode.The electrochemical characteristics of SN were studied by cyclic voltammetry（CV） and differential pulse voltammetry（DPV） in a Britton-Robinson（B-R） buffer solution.Results：Under optimum experimental conditions,the anodic peak current obtained by DPV was linearly dependent on SN concentration in the range of 5.0×10-7-1.0×10-4 mol·L-1.The detection limit was 2.2×10-7 mol·L-1.Conclusion：The modified electrode is inexpensive,easy to prepare with fewer sample volume and strong anti-interference and is suitable for the determination of SN in Zhengqing Fengtongning injection samples.

关 键 词：青藤碱电化学性质 石墨烯 纳米金 印刷电极 循环伏安法(CV) 示差脉冲伏安法(DPV) 

分 类 号：R917[医药卫生—药物分析学]