QuEChERS-UPLC-MS/MS快速测定猪肝中20种磺胺类药物残留  

UPLC-MS/MS via QuEChERS rapid determination of twenty sulphonamide residues in pig liver

作　　者：曲斌[1] 朱志谦[1] 陆桂萍[1] 蒋天梅[1] 耿士伟[1] 吴玲[1] 

机构地区：[1]江苏省畜产品质量检验测试中心,南京210036

出　　处：《药物分析杂志》2012年第8期1457-1464,共8页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立了一种以QuEChERS方法做为样品前处理技术,超高效液相色谱-串联质谱法快速测定猪肝中20种磺胺类药物残留量的方法。方法:猪肝样品以磺胺二甲氧嘧啶-D6为内标,经DisQuE萃取管用乙腈提取,DisQuE净化管净化,浓缩后经超高效液相色谱分离,三重串联四极杆质谱法测定。结果:在10～200μg.kg-1范围内各磺胺类药物均呈良好的线性关系,高中低浓度回收率在70%～115%,精密度小于15%。结论:本方法快速、简便,适用于猪肝中磺胺类药物的多残留批量测定。Objective：To establish an ultra performance liquid chromatography tandem mass spectrometric method for rapid determination of twenty sulphonamides residues in pig liver via QuEChERS.Method：Sulfadimethoxine-D6 was adopted as internal standard.The sample was extracted by acetonitrile in DisQuE extraction tube,cleaned up by DisQuE clean-up tube.After concentration,it was analyzed by UPLC-MS/MS.Results：The results showed that good linearity in the ranges of 10-200 μg·kg-1 for each sulphonamide component.The recoveries were between 70% and 115%,and precision was lower 15%.Conclusion：The method is simple and rapid,and it is suitable to detect sulphonamides multi-residuals with high throughput.

关 键 词：QuEChERS样品前处理 超高效液相色谱串联质谱 食品抗生素药物残留检测 

分 类 号：R917[医药卫生—药物分析学]