猪体内筛选RHDV VP60 T细胞表位的优势区  

Screening specific T lymphocyte epitopes superiority region of VP60 protein of rabbit hemorrhagic disease virus in pig

作　　者：金明兰[1,2] 侯继波 郑其升 鲁会军[2] 任静强[2] 南文龙[2] 韩松[2] 金宁一[2] 

机构地区：[1]国家兽医生物制品工程技术研究中心,江苏南京210014 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国兽医学报》2012年第8期1089-1093,共5页Chinese Journal of Veterinary Science

基　　金：江苏省博士后科研启动基金资助项目(20080645)

摘　　要：应用戊二醛醛化的人"O"型红细胞纯化兔出血症病毒(RHDV),对非免猪进行3次免疫后,分离外周血淋巴细胞经不同段重组杆状病毒(共分5段表达)、纯化的病毒、Con A刺激后进行IFN-γ、IL-2、IL-4、WST检测。结果显示,纯化病毒的血凝效价为28,与未纯化的RHDV毒价相差1个滴度;WST检测结果为免疫组经3段重组杆状病毒刺激的值最高;IFN-γ和IL-2检测结果也是3段重组杆状病毒刺激的值最高;IL-4检测结果为3段重组杆状病毒刺激的值最低。由此表明病毒经纯化后效果好,3段重组杆状病毒区域为T细胞表位优势区,这为RHDV T细胞表位的进一步研究奠定基础。The pigs were inoculated three times with the rabbit hemorrhagic disease virus（RHDV） purified by prepa- ration of hydroformylated human （Ytype red blood ceil to detect IFN-γ, IL-2, IL-4, WST after sterility isolation blood lymphocyte stimulated by various rebaculoviruses, purified RHDV and Con A. The hemagglutination valency of puri- fied virus was 28 ,in correspondence with the unpurified virus. The statistically analysis was performed using SPSS software packages. The NO. 3 rebaculoviruses was the highest quantities of WST producing than the others （P〈 0.01）. In addition, the NO. 3 rebaculoviruses was the highest quantities of IFN-γ and IL-2 producing than the oth- ers,but was the lowest quantities of IL-4 producing than the others. It is demonstrated that efficiency of purified RHDV was high and the NO. 3 rebaculoviruses contain T-cell epitopes.

关 键 词：猪 兔出血症病毒 VP60 T细胞表位 IFN-Γ IL-2 IL-4 WST 

分 类 号：S852.65[农业科学—基础兽医学]