牛病毒性腹泻病毒感染牛外周血单核细胞对IFN-α、β、γmRNA转录的影响  

Effects of bovine viral diarrhea viruses in vitro on transcription of interferon-alpha,beta,gamma mRNA in bovine peripheral blood mononuclear cells

作　　者：韩猛立[1] 黄新[1] 钟发刚[1] 

机构地区：[1]新疆农垦科学院新疆生产建设兵团绵羊繁育生物技术重点实验室,新疆石河子832000

出　　处：《中国兽医学报》2012年第8期1110-1115,共6页Chinese Journal of Veterinary Science

基　　金：国家"973"计划前期研究专项资助项目(2008CB117017);国家自然科学基金资助项目(30960286);新疆农垦科学院青年科学基金资助项目(YQJ201113)

摘　　要：为了解牛病毒性腹泻病毒(BVDV)感染对干扰素(IFN)mRNA转录时相的影响,探讨宿主-病毒之间的相互关系,用非致细胞病变(noncytopathic,NCP)和致细胞病变(cytopathic,CP)型BVDV感染临床健康BVDV检测阴性的荷斯坦奶牛外周血单核细胞(PBMC),利用实时荧光定量PCR技术对感染后IFN-α、β、γmRNA转录水平的变化进行定量分析。结果表明,CP型和NCP型BVDV感染PBMC后,Ⅰ型IFN(IFN-α、β)均呈现出不同程度的转录水平上调,且差异极显著(P<0.01);只有IFN-α在CP型BVDV感染后4,12h(P<0.5)出现转录下调。IFN-γ在整个感染过程中均呈现出不同程度的转录水平上调,且差异显著(P<0.05)。这表明2种生物型BVDV感染可引起PBMC中IFN mRNA转录水平升高。The study was done to survery the interferon-alpha, beta and gamma mRNA transcription profiles of bovine viral diarrfea viruse（BVDV） infection,and to investigate the host-BVDV interaction. The clinically healthy Holstein cows tested negative for bovine viral diarrhea virus（BVDV） in peripheral blood mononuclear cells（PBMC） were in- fected with noncytopathic（NCP） and cytopathic（NCP） BVDV. The mRNA levels of IFN-α,β and γ genes were ana lyzed using a reaPtime fluorescent quantitative PCR（reaPtime FQ-PCR）. The results indicated that the transcription of I type（IFN-α,β） mRNA showed a different increasing levels （P〈0.01） ,after infected CP- and NCP BVDV in PBMC;only IFN-α decreased at 4,12 h（P〈0. 05） after infected CP-BVDV. And IFN-γ was increased throughout the infection process of CP and NCP BVDV in PBMC （P〈0. 05）. The transcription levels of IFN mRNA were in- creased when two biotype of BVDV infected in PBMC.

关 键 词：牛病毒性腹泻病毒 外周血单核细胞 干扰素 实时荧光定量PCR 

分 类 号：S852.65[农业科学—基础兽医学]